两组中药方剂对糖尿病足产ESBLs大肠埃希菌和MRSA体外药物敏感性试验结果分析  

Analysis on the in vitro susceptibility test results from two groups of traditional Chinese medicine prescriptions for diabetic foot producing ESBLs Escherichia coli and MRSA

作　　者：梁晓谊 朱海英 刘笑梅 潘敏 

机构地区：[1]华佗医院检验科,广东肇庆526040

出　　处：《检验医学》2015年第3期261-264,共4页Laboratory Medicine

摘　　要：目的了解黄连液和黄连液联合麝香2组方剂对糖尿病足常见多重耐药菌——产超广谱β-内酰胺酶（ESBLs）大肠埃希菌和耐甲氧西林金黄色葡萄球菌（MRSA）的抑菌和治疗效果。方法收集确诊为糖尿病足的患者,经细菌培养分离出病原菌138株,筛选出其中的产ESBLs大肠埃希菌和MRSA共67株,运用琼脂稀释法检测中药组方一（黄连液）及组方二（黄连液联合麝香）对2种病原菌的最小抑菌浓度（MIC）。同时观察2组方剂对糖尿病足的治疗效果。结果组方一对35株产ESBLs大肠埃希菌的MIC≥128 mg/m L,而组方二其MIC值下降至32 mg/m L,组方二的MIC值范围为32-128 mg/m L。32株MRSA的MIC值也从组方一的32 mg/m L降至组方二的8 mg/m L。经比较发现,加入麝香的组方二对2种细菌的MIC值较组方一下降明显,并且疗效显著,差异有统计学意义（P〈0.05）。结论用中药组方黄连液联合麝香对糖尿病足常见的病原菌——产ESBLs大肠埃希菌和MRSA具有显著的抑菌作用,这为今后的临床治疗提供了一种新的方法。Objective To understand the inhibitory and treatment effects of Coptis chinensis and Coptis chinensis with Musk prescriptions for diabetic foot producing extended-spectrum beta lactamase（ESBLs） Escherichia coli and methicillin-resistant Staphylococcus aureus（MRSA）.Methods The patients diagnosed as diabetic foot were enrolled.After culturing,138 isolates of bacteria were isolated,and 67 isolates of ESBLs Escherichia coli and MRSA were screened.The minimum inhibitory concentrations（MIC） of Coptis chinensis（prescription 1） and Coptis chinensis with Musk（prescription 2） were determined by agar dilution method.The treatment effect of the 2 groups of prescriptions for diabetic foot was observed.Results The MIC of prescription 1 for 35 isolates of ESBLs Escherichia coli was ≥128mg / mL,and the MIC of prescription 2 was 32mg / mL.The MIC scope of prescription 2 was 32-128mg / mL.The MIC for 32 isolates of MRSA droped from 32mg / mL（prescription 1） to 8mg / mL（prescription 2）.Compared with prescription 1,the MIC of prescription 2 decreased significantly,the treatment effect was distinct,and the differences were statistically significant（P〈0.05）.Conclusions Traditional Chinese medicine prescription Coptis chinensis with Musk has inhibitory and treatment effects for diabetic foot producing ESBLs Escherichia coli and MRSA,which is a novel method for clinical treatment.

关 键 词：中药敏感性试验 超广谱Β-内酰胺酶 大肠埃希菌 耐甲氧西林金黄色葡萄球菌 黄连液 麝香 糖尿病足 

分 类 号：R446.5[医药卫生—诊断学]