PCR-核糖体分型技术基因分型艰难梭菌  

PCR-ribotyping for genotyping Clostridium difficile clinical isolates

作　　者：肖克林[1] 金萍[2] 黄丽清[3] 梁霞[4] 周天祥[3] 王中兴[3] 王秦宁[5] 孔繁荣[5] 

机构地区：[1]深圳市宝安区妇幼保健院中心实验室,广东深圳518133 [2]深圳市宝安区妇幼保健院儿科ICU,广东深圳518133 [3]深圳市宝安区妇幼保健院检验科,广东深圳518133 [4]深圳市宝安区妇幼保健院药剂科,广东深圳518133 [5]悉尼大学韦斯特米德医院感染性疾病和微生物中心

出　　处：《国际检验医学杂志》2015年第6期793-795,共3页International Journal of Laboratory Medicine

基　　金：宝安区科技计划项目(2013071;2014083)

摘　　要：目的调查区域性的艰难梭菌基因型分布和毒素A、B的携带情况。方法 68株艰难梭菌临床菌株收集自悉尼大学韦斯特米德医院,利用PCR-核糖体分型技术进行基因分型,利用PCR方法检测毒素A、B编码基因tcdA、tcdB。结果 68株菌可分为31种核糖体型(RT),最常见的为RT014(19.1%)和RT002(11.8%);94.1%(64株)的菌株tcdA、tcdB均为阳性。结论悉尼地区主要流行的艰难梭菌基因型为RT014和RT002,绝大部分临床菌株均携带毒素A、B。Objective To investigate the genotype and production of toxin A and B of C .difficile clinical isolates collected from Sydney ,Australia .Methods Sixty‐eight C .difficile clinical isolates were collected from Westmead Hospital ,the University of Sydney ,which were genotyped by using PCR‐ribotyping ,and toxin A ,B coding gene tcdA ,tcdB were detected by using PCR meth‐od .Results Thirty‐one PCR‐ribotypes （RTs） were confirmed in the 68 C .difficile clinical isolates ,RT014 （19 .1% ） and RT002 （11 .8% ） were the common genotypes .Sixty‐four of 68 （94 .1% ） isolates contained tcdA and tcdB for toxin A and B .Conclusion The common prevalent PCR‐ribotypes of C .difficile were RT014 and RT002 in Sydney ,most of the C .difficile clinical isolates contained toxin A and B .

关 键 词：艰难梭菌 核糖体型 毒素A、B 

分 类 号：R446.5[医药卫生—诊断学]