HBeAg转阴前后慢性乙肝患者HBV cccDNA荧光定量检测的意义  

Significance of fluorescence quantitative detection of HBV cccDNA in patients with chronic hepatitis B before and after HBeAg seroconversion

作　　者：王志剑 郭振华[2] 

机构地区：[1]河源市源城区人民医院检验科,广东河源517000 [2]兰州大学第一医院检验科,甘肃兰州730000

出　　处：《国际检验医学杂志》2015年第6期806-807,810,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨慢性乙型肝炎患者乙型肝炎病毒(HBV)cccDNA检测的意义。方法检测乙型肝炎患者乙肝e抗原(HBeAg)转阴前后肝组织HBV共价闭合环状DNA(cccDNA)、血清HBV DNA。用实时荧光定量PCR方法检测HBV DNA、cccDNA水平,通过质粒保护型的DNA酶对提取的DNA模板进行酶切处理,再分别进行HBV DNA和cccDNA的定量检测。结果HBeAg转阴前,慢性乙肝患者总HBV DNA水平和cccDNA水平呈正相关(r=0.911,P<0.01);而HBeAg转阴后,血清HBV DNA水平和肝组织cccDNA水平的检测不一致。结论 HBV cccDNA可作为反映慢性乙肝患者抗病毒治疗预后的指标。Objective To explore the significance of anti‐viral marker cccDNA detection in the patients with chronic hepatitis B . Methods Serum hepatitis B virus（HBV） DNA and covalently closed circular DNA （cccDNA） in liver tissue were detected before and after HBeAg seroconversion in the patients with hepatitis B by the real time fluorescent PCR method .The extracted DNA tem‐plates were digested by the protective plasmid DNA enzyme .Then HBV DNA and cccDNA were detected .Results The total HBV DNA level was positively correlated with the cccDNA level before HBeAg negative conversion in the patients with chronic hepatitis B ,the detection of serum HBV DNA level and liver tissue cccDNA level was inconsistent .Conclusion HBV cccDNA may serve as a prognostic indicator reflecting the antiretroviral therapy in the patients with chronic hepatitis B .

关 键 词：慢性乙肝 共价闭合环状DNA 乙肝E抗原 

分 类 号：R512.62[医药卫生—内科学]