红嘴相思鸟热应激及壳聚糖抗应激效应研究  

Studies on Heat Stress and Effect of Chitosan Anti-stress of Leiothrix lutea

作　　者：刘明[1] 王夕龙[1] 袁施彬[1] 周材权[1] 

机构地区：[1]西华师范大学生命科学学院珍稀动植物研究所,西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2015年第1期1-6,11,共7页Journal of China West Normal University(Natural Sciences)

基　　金：国家自然科学基金(31470462);西华师范大学西南野生动植物资源保护教育部重点实验室开放基金项目(XNYB11-4)

摘　　要：为了探讨人工驯繁条件下高温对红嘴相思鸟Leiothrix lutea生理机能的影响及壳聚糖的抗应激效应,本研究采用单因子实验设计,将80只成年健康红嘴相思鸟(♀)随机分到5个处理中(处理1为常温对照组,处理2、3、4、5为急性高温应激组,处理温度35℃,持续3h),分别在其基础日粮中添加0%,0%,0.1%,0.5%,和1%的壳聚糖,检测血浆中Ig G、Ig M和Ig A的浓度,组织中抗氧化酶活力和脂质过氧化指标丙二醛(MDA)的含量.结果发现:与常温对照组相比,各热应激对照组红嘴相思鸟免疫球蛋白(除Ig A)含量降低,体内抗氧化酶活力下降(3种组织中T-SOD酶活均显著降低,肝脏中GSH-PX及CAT酶活显著降低),脂质过氧化产物含量升高(肝脏和肌肉中MDA含量显著升高);而壳聚糖又能不同程度的提高免疫球蛋白(除Ig A)的含量,增强体内抗氧化酶活力,缓解体内脂质过氧化状况,且壳聚糖的添加剂量在0.5%时,机体表现出最佳的抗应激能力.In order to explore the effect of heat stress on the physiological function of red billed Leiothrix（Leiothrix lutea）, and the anti-stress effect of chitosan, we used single factor experiment design to assign 80 healthy adult fe- male red billed Leiothrixs to the five treatments,randomly（Treatment 1 is the normal control group,treatment 2,3, 4,5 are acute heat stress group,treatment temperature is 35~C for 3 h）. And we added 0% ,0% ,0.1% ,0.5% ,and 1% of chitosan to the basic diet, respectively, to detect the concentration of IgG, IgM and IgA in plasma, the antioxi- dant enzyme activity in organization and the content of lipid peroxide malondialdehyde（MDA）. The results showed that：the concentration of immunoglobulin （except IgA） decreased in the control group of heat stressed red billed Leiothrix,comparing with the normal temperature control group;the activity of anti-oxidative enzyme decreased（the activity of T-SOD was decreased significantly in three kinds of tissue ,the activity of GSH-PX and CAT were signifi- cantly reduced in liver）, the level of lipid per oxidation was increased （the content of MDA increased significantly in the liver and muscle） ;chitosan can improve the level of immune globulin to a variable extent（ except for IgA） , in- crease the activity of anti-oxidative enzyme, alleviate the status of lipid per oxidation, and the body of red billed Leiothrix showed the best anti-stress ability,when we added 0.5% of chitosan.

关 键 词：红嘴相思鸟 壳聚糖 热应激 抗应激 

分 类 号：Q958.14[生物学—动物学]