东北菱粗多糖的提取和纯化方法的评价  

Evaluation on extraction and purification of raw polysaccharide in northeast water chestnut

作　　者：刘阳[1] 张岩[2] 谢文兵[3] 伍翔群[1] 郭建[1] 迪拉热.力迪甫 牛凤兰[1] 

机构地区：[1]吉林大学公共卫生学院卫生检验学教研室,吉林长春130021 [2]吉林大学第一医院耳鼻咽喉-头颈外科,吉林长春130021 [3]中国科学院长春应用化学研究所国家电化学和光谱研究分析中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2015年第2期420-424,共5页Journal of Jilin University：Medicine Edition

基　　金：国家重点基础研究发展计划(973计划)项目资助课题(2011CB935800);吉林省科技厅重点项目资助课题(20070424)

摘　　要：目的:研究提取东北菱多糖的最佳溶剂,比较4种多糖纯化方法对东北菱粗多糖的纯化效果。方法:将东北菱粗提物分为3份,分别用甲醇、乙醇和丙酮作为溶剂提取粗多糖。分别采用Sevag法、三氯乙酸法、盐酸法和活性炭法对东北菱粗多糖进行脱蛋白处理。用比色法检测东北菱提取物中多糖水平和纯化后样品中多糖水平和蛋白质水平,比较纯化效果。结果:3种提取物中,以丙酮为溶剂的东北菱提取物中多糖水平最高,为33.69%,蛋白质水平为5.51%。经Sevag法、三氯乙酸法、盐酸法和活性炭法处理后的溶液中多糖水平分别为46.53%、43.44%、41.24%和36.66%;蛋白水平分别为5.64%、5.41%、5.41%和5.51%。结论:丙酮为提取东北菱粗多糖的最佳溶剂。4种纯化方法各有优缺点,Sevag法对于多糖水平的提高效果明显,适合东北菱粗多糖的提取。Objective To select the best extraction solvent of the raw polysaccharide in northeast water chestnut,and to compare the purify effects of 4kinds of purification methods for raw polysaccharide.Methods The raw extracts from northeast water chestnut were divided into three parts.The raw polysaccharide in northeast water chestnut was extracted with methanol,ethanol,and acetone. The polysaccharide of water-chestnut was deproteinized by Sevag,trichloroacetic acid,hydrochloric,and activated carbon methods. The levels of polysaccharide and protein were detected by colorimetric method,and their purification effects were compared.Results The highest level of the polysaccharide in the water-chestnut extracts was obtained by using acetone as the extraction solvent;the level of polysaccharide was 33.69% and the level of protein was 5.51%.After treated by Sevag,trichloroacetic acid,hydrochloric,and activated carbon,the levels of polysaccharides were 46.53%,43.44%,41.24%,and 36.66%;the levels of proteins were 5.64%,5.41%,5.41%,and 5.51%,respectively.Conclusion Acetone is the best extraction solvent of the raw polysaccharide of northeast water chestnut.The four purification methods have advantages and disadvantages,and the highest polysaccharide level is obtained by Sevag method,and it is suitable for extraction of raw polysaccharide in northeast water chestnut.

关 键 词：东北菱 提取 纯化 多糖 蛋白质 

分 类 号：R284.2[医药卫生—中药学]