红景天苷对大鼠肠系膜动脉舒缩作用及调控机制的研究  

Vasomotor Effect of Salidroside on Rat Mesenteric Artery and Its Regulatory Mechanisms

作　　者：孙卫卫[1] 徐鹏[2] 崔英凯[2] 刘海燕[2] 曹雪滨[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]解放军第252医院心内科,河北保定071000

出　　处：《中国中医基础医学杂志》2015年第3期345-347,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：全军医学科技"十二五"科研项目及重点项目(13WS11J058)-部队官兵应激情况下重要器官损伤的预警与综合防治;后勤科研项目;面上项目(CBJ13J006)-力竭心脏损伤的钙通道调控机制和药物干预作用的研究

摘　　要：目的:探讨红景天苷(salidroside,SAL)对大鼠离体肠系膜动脉的舒缩作用及调控机制。方法:应用DMT微血管张力测定法和ELISA法研究红景天苷对血管的影响及机制。结果:SAL对KCl预处理的血管环起收缩作用;对PE预处理的血管起舒张作用,其中内皮完整血管比去内皮血管舒张明显;L-NAME抑制SAL舒血管,亚甲蓝、吲哚美辛对SAL舒血管无影响;SAL升高血管NO和NOS。结论:SAL对血管具有收缩和舒张双重作用,缩血管可能与电压依赖性钙通道有关;舒血管可能与抑制内钙释放有关,具有与NO释放和NOS合成有关的内皮依赖性,但与环氧合酶途径无关。Objective： To observe vasomotor effect of salidroside（ SAL） on rat mesenteric artery,and to explore its underlying regulatory mechanisms. Method： Tension was measured by DMT system to evaluate the vasomotor effect of SAL on rat endothelium-intact and endothelium-denuded mesenteric aortic rings. Different antagonists and ELISA method were used to illustrate the mechanisms of vasomotor effect of SAL. Result： 1 SAL had significantly vasoconstrictive effect on mesenteric aortic rings precontracted by KCl. 2 SAL relaxed endothelium-intact and endothelium-denuded mesenteric artery precontracted by PE in concentration-dependent manner,while the vasodilation of endothelium-intact mesenteric artery was significantly stronger than that of endothelium-denuded one. 3L-NAME attenuated the vasodilation of endothelium-intact mesenteric artery induced by SAL,while neither indomethacin nor methylene blue affected it. 4 Using ELISA,SAL can significantly increase NO and NOS of mesenteric artery. Conclusion： The results indicate that SAL plays a dual role of vasoconstriction and vasodilation in regulating mesenteric artery. Vasoconstriction may be associated with voltage-dependent calcium channel,while vasodilation may be associated with inhibition of intracellular calcium release. Besides,vasodilation shows endothelium dependence,which is related to NO released and NOS synthesized,regardless of the COX pathway.

关 键 词：红景天苷 肠系膜动脉 NO CA2+ DMT系统 

分 类 号：R285.6[医药卫生—中药学]