正交试验法优选蒺藜总皂苷和总黄酮的提取工艺  

Optimization of Extracting Procedure of Total Saponins and Total Flavonoids from Tribulus terrestris L by Orthogonal Test

作　　者：刘李梅[1] 杨明[2] 郭志烨 梅明[1] 韩丽[1] 邹文铨[3] 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [3]四川大学,四川成都610064

出　　处：《现代中药研究与实践》2015年第1期44-47,共4页Research and Practice on Chinese Medicines

摘　　要：目的优选蒺藜总皂苷、总黄酮的提取工艺。方法采用正交试验法对提取工艺条件进行优化,以氯化钴作为对照品检测蒺藜中总皂苷;芦丁作为对照品检测蒺藜中总黄酮。结果最优提取工艺为10倍水提取3次,每次1.5h。蒺藜中呋甾皂苷含量为1.7%,蒺藜总黄酮含量0.44%。结论该工艺经济、可行。Objective To select the optimal extracting procedure for total saponins and total fl avonoids from Tribulus terrestris L. Methods The procedure was selected with the contents of total saponins and total fl avonoids from Tribulus terrestris L. by orthogonal test design. Results Optimum extraction process was using ten fold water,3 times,1.5hours per. The contents of total saponins was 1.7%, total fl avonoids was 0.44%. Conclusion The process is economic and feasible.

关 键 词：蒺藜 总皂苷 总黄酮 正交试验 

分 类 号：R284.2[医药卫生—中药学]