苦参中生物碱类成分的稳定性及减压提取工艺研究  

The Stability of Alkaloids in Sophora fl avescens and Vacuum Extraction Technology Research

作　　者：梅明[1] 伍振峰[1,2] 韩丽[1] 张定堃[1] 杨明[1,2] 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用国家重点实验室培育基地,四川成都611137 [2]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《现代中药研究与实践》2015年第1期48-51,共4页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金项目(81173565)

摘　　要：目的考察苦参中有效成分苦参碱与氧化苦参碱的稳定性,并对苦参的减压水提工艺参数进行优化。方法采用高效液相色谱法,研究苦参碱与氧化苦参碱在混合对照品溶液及苦参饮片减压提取供试品中随温度和时间的变化规律;利用单因素考察结合正交试验设计优化苦参减压水提工艺条件。结果苦参碱与氧化苦参碱单体混合溶液及苦参减压提取供试品在不同温度下含量变化微小。苦参提取过程中提取温度会影响苦参碱与氧化苦参碱的总量和比值;苦参减压水提取的最优工艺参数为提取温度70℃,提取时间1h,提取次数为3次,液料比为8:1。结论苦参碱和氧化苦参碱单体稳定性良好,但苦参饮片在提取过程中由于其它成分的影响,氧化苦参碱会向苦参碱转化。减压提取适合此类中药材的提取,苦参减压水提取工艺合理、稳定、可行,可为其合理利用提供参考。Objective To investigate the stability of matrine and oxymatrine in solution and to optimize the process parameters in vacuum extraction of Sophora. Methods Using the method of HPLC to study the regular pattern of matrine and oxymatrine in mixed standard solution and Sophora vacuum water extraction sample varied with temperature and time; using single factor experimental design and orthogonal design to optimize sophora vacuum water extraction process conditions. Results Change at different temperatures of matrine and oxymatrine monomer mixed solution and Sophora extracted samples were small. Sophora extraction temperature could affect the total and ratio of matrine and oxymatrine in the extraction process; the optimal process parameters of Sophora vacuum water extraction was extraction for 3 times with temperature was 70℃, 1h for each time, the ratio of liquid to solid is 8：1. Conclusion Matrine and oxymatrine monomer have good stability, but Sophora in the extraction process due to the infl uence of other components, oxymatrine would transfer to matrine. Vacuum extraction was suitable for this kind of traditional Chinese medicine, Sophora vacuum water extraction technology was reasonable, stable, feasible and can provide reference for the rational use.

关 键 词：苦参碱 氧化苦参碱 稳定性 减压提取 正交设计 

分 类 号：R284.2[医药卫生—中药学]