2型糖尿病患者口腔念珠菌负荷与唾液HBD-2及HBD-3水平的初步研究  

PRIMARY STUDY OF SALIVARY DEFENSINS EXPRESSIONS AND ORAL CANDIDOSIS EXPERIENCE IN NON-INSULIN-DEPENDENT DIABETES MELLITUS

作　　者：张林林[1] 陈波[1] 陶人川[1] 马飞[1] 

机构地区：[1]广西医科大学附属口腔医院牙周黏膜科,南宁530021

出　　处：《广西医科大学学报》2015年第1期41-44,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30860313);广西壮族自治区科技厅科技攻关与新产品试制资助项目(No.桂科攻114003A28)

摘　　要：目的:探讨2型糖尿病患者的口腔念珠菌感染情况及其唾液人类β防御素(HBD-2、HBD-3)的表达水平和相互关系。方法:纳入确诊6个月以上近期病情没有变化的2型糖尿病患者39例为实验组及健康者40例为对照组,分别采集两组未刺激全唾液、含漱液,进行口腔念珠菌培养鉴定计数,并采用酶联免疫吸附法检测唾液HBD-2及HBD-3表达水平。结果:实验组中27例(69.2%)念珠菌培养呈阳性,明显高于对照组(20.0%),均以白色念珠菌为主。实验组唾液HBD-2和HBD-3的水平中位数分别为0.045μg/L和8.061μg/L,均明显高于对照组(0.024μg/L和0.832μg/L),两组比较差异均有统计学意义(P<0.05);口腔念珠菌负荷与唾液HBD-3的水平呈正相关(rs=0.245,P<0.05);唾液HBD-2与HBD-3的水平呈正相关(rs=0.370,P<0.01)。结论:2型糖尿病患者的口腔念珠菌负荷普遍较高,分离株以白色念珠菌为主,其唾液中HBD-2、HBD-3的水平均升高,并与口腔念珠菌负荷呈正相关关系。Objective： The aim of this study was to explore the levels of HBD-2 and HBD-3 protein in saliva of NIDDM patients and their relationship with oral candidiasis. Methods： 39 NIDDM patients who were di- agnosed for over 6 months without recent fluctuation and 40 healthy controls were recruited. Oral rinse and unstimulated saliva were collected. Oralcandida were cultured and calculated and the concentration of HBD 2,-3 were detected by enzyme-linked immunosorbent assay. Results：In NIDDM group, oralcandida were i- solated from 27 of the 39 patients （69.2%）, which were mainly identified as C. albicans. While in healthy controls, only 20.0G of oral candida culture was positive. The median concentrations of HBD-2 and HBD- 3 in NIDDM group were 0. 045μg/L and 8. 061 μg/L, both higher than that of healthy controls （0. 024μg/ L and 0. 832μg/L, P〈0.05）. The concentrations of HBD-3 were correlated positively with oral candida carriage （ rs =0. 245, P〈0.05）. The concentrations of HBD-2 and HBD-3 were also correlated positively （ rs = 0. 370, P〈0.01）. Conclusion： A higher prevalence of oral candidiasis and highercandida carriage were found in NIDDM patients compared with healthy controls. C. albicans was the most common isolated species. The levels of HBD-2, -3 in saliva were both up-regulated in NIDDM patients and were correlated positively with oral candida carriage.

关 键 词：2型糖尿病 口腔念珠菌 人类β防御素-2 人类β防御素-3 

分 类 号：R379.4[医药卫生—病原生物学]