N-糖基化猪瘟病毒E2蛋白不同接种剂量对BALB/c小鼠的免疫效果分析  

Analysis of immune effect of N-glycosylated E2 protein of classical swine fever virus with different inoculation doses in BALB/c mice

作　　者：孙全文[1] 王爱华[1] 董炳梅 杨翠军[1] 王金良[2,3] 

机构地区：[1]河北北方学院动物科技学院,河北张家口075051 [2]山东绿都生物科技有限公司,山东滨州256600 [3]山东省滨州畜牧兽医研究院,山东滨州256600

出　　处：《中国兽医学报》2014年第8期1225-1230,共6页Chinese Journal of Veterinary Science

基　　金：山东省自然科学基金资助项目(ZR2010CQ012);河北北方学院重大课题资助项目(ZD201307);河北省2014年科技攻关资助项目(14226604D)

摘　　要：E2蛋白(E2 protein,E2-Pro)为猪瘟病毒(classical swine fever virus,CSFV)的主要结构蛋白之一,是CSFV最主要的保护性抗原,因此应用体外糖基化技术获得的N-糖基化E2蛋白(glycosylated E2 protein,Gly-E2-Pro)理论上可进一步增强机体的免疫应答。为了验证这一设想以及获得最佳的免疫效果,本研究分别将Gly-E2-Pro按20,50,100μg/只,接种BALB/c小鼠,同时设E2-Pro与CSFV对照,于不同时间采血制备血清,通过血清中IgG、IL-4及INF-γ水平的测定,评价Gly-E2-Pro的免疫效果及确定最佳免疫剂量。结果显示,免疫Ⅰ组(20μg/只)Gly-E2-Pro虽刺激机体产生了IgG免疫应答,但水平较低,与E2-Pro、CSFV组差异不显著;免疫Ⅲ组(100μg/只)Gly-E2-Pro免疫后,前期出现免疫抑制,至28d抗体水平急剧上升,达到极高值;免疫Ⅱ组(50μg/只)Gly-E2-Pro免疫后,血清IgG水平显著高于E2-Pro与CSFV组,同时显著高于免疫Ⅰ组与Ⅲ组。以上结果说明,Gly-E2-Pro可有效增强机体的体液免疫应答,50μg/只为最佳接种剂量。E2 protein（E2-Pro） is one of the major structural proteins of the classical swine fever vi- rus（CSFV）. It is the major protective antigen of CSFV. The N-glycosylated E2 protein（Gly-E2- Pro） which had been glycosylated in vitro will enhance the immune response in theory. Therefore, in order to verify this idea and acquire the best immunity effect, the BALB/c mice were inoculated with 20,50,100 μg respectively and the serum was prepared at different time,the E2-Pro and cSFV as control at the same time. Sequentially,the immune effect and the most optimum immune dose of Gly-E2-Pro were evaluated through the determination of IgG, IL-4 and INF-7 levels in serum. The results showed that the Gly-E2-Pro induced immune responsed, but the IgG levels decreased in the group I （20 μg）, but not significantly different with the E2-Pro and CSFV. In the group Ⅲ （100 μg）,the immune suppression was observed from 7 d to 21 d after inoculation,and the IgG levels sharply rised and achieved a maximum value on the 28 d. Gratifyingly,in the group Ⅱ（50μg） ,the IgG levels in serum were significantly higher than that of E2-Pro and CSFV after Gly-E2-Pro inoculated. The above results showed that the Gly-E2-Pro can effectively enhance the humoral immune response and the optimum dosage was 50μg.

关 键 词：BALB/C小鼠 N-糖基化 猪瘟病毒 E2蛋白 接种剂量 免疫效果 

分 类 号：S852.65[农业科学—基础兽医学]