马立克氏疫苗病毒在鸡传代细胞上的培养工艺  

Culturing technology of Marek's disease virus grown in a continuous cell line from chickens

作　　者：刘玉鹏 赖汉漳 李延鹏 罗琼 陈瑞爱[1,2] 

机构地区：[1]农业部动物疫病防控生物技术与制品创制重点实验室广东省兽用生物制品技术研究与应用企业重点实验室肇庆大华农生物药品有限公司,广东肇庆526238 [2]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医学报》2014年第8期1244-1247,1252,共5页Chinese Journal of Veterinary Science

摘　　要：为探索马立克氏病(Marek's disease,MD)疫苗病毒在DF-1细胞系上的最佳培养工艺以及由此方法制备的马立克氏病疫苗对鸡的保护效力,通过进行培养基和血清选择、病毒在DF-1细胞上接种稀释度和接种方式选择以及滴度测定,并制备以DF-1细胞为基质的CVI988和FC-126病毒疫苗,按中华人民共和国兽用生物制品规程(2000)检验合格后与其他商品苗进行保护效率对比试验。结果表明,试验成功探索了CVI988和FC-126病毒在DF-1细胞系上的培养工艺,以DF-1细胞为基质制备的疫苗也取得了和其他商品苗保护率相当的良好效果,因此推断DF-1细胞可以作为马立克氏病疫苗病毒的传代细胞培养候选基质。This study aimed to find the optimal culturing technology of Marek＇s disease （MD）virus grown in DF-1 cell line,and test the protection index（PI） of MD vaccines which used DF-1 cells as the substate. After the culture media and serum were selected for DF-1 cell line,the optimal dilution and inoculation way were selected for the MD virus and tested titres of the virus grown in DF- 1 cells,and then we tested the PI of the CVI988 and the FC-126 vaccines which used DF-1 cells as the substate with other commercial vaccines,which amply complied with the requirements of the PRC Animal Biologicals Procedures （2000）. This study tentatively researched the culturing technology of the CVI988 and the FC-126 virus grown in DF-1 cells,and the PI of the vaccines were similar to other commercial vaccines. These results indicated that DF-1 cell line is a suitable candidate substate for the current vaccines against MD.

关 键 词：马立克氏病病毒 DF-1细胞系 培养工艺 保护指数 

分 类 号：S852.65[农业科学—基础兽医学]