无血清培养基诱导破骨样细胞分化  

Osteoclast-like-cell differentiation in serum-free conditions

作　　者：付应霄[1,2] 顾建红[1] 袁燕[1] 刘学忠[1] 卞建春 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]蚌埠医学院生物科学系,安徽蚌埠233030

出　　处：《中国兽医学报》2014年第8期1309-1313,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31172373;31372495;31302154);江苏省高校优势学科建设工程资助项目;教育部博士点基金资助项目(20113250110003)

摘　　要：探索了无血清培养基对RAW264.7细胞诱导破骨样细胞(osteoclast-like-cell,OCL)分化的影响。结果显示,无血清条件下,M-CSF+RANKL能够诱导RAW264.7细胞分化产生TRAP阳性OCL,F-actin及Hoechst33258染色观察到OCL骨架结构完整、核规则,具有骨吸收活性。这表明在无血清条件下RAW264.7细胞可诱导分化产生OCL,但其存活时间短,此外,M-CSF在其分化过程中具有不可替代的作用。This study explored the effects of serum-free medium on RAW264.7 cells induced osteoclast-like-cell （OCL） differentiation. The results elucidated that M-CSF＋RANKL could induce TRAP positive OCL in serum-free conditions;F-actin staning and Hoechst33258 staining showed that the OCL characterized multiple nuclei and intact cytoskeleton structure and possessed bone resorption activity. In summary, OCL should be induced from RAW264.7 cells in serum-free conditions, however,its lifespan was short, furthermore, M-CSF was indispensable in osteoclastogenesis

关 键 词：无血清培养 RAW264.7细胞株 破骨样细胞 

分 类 号：S852.1[农业科学—基础兽医学]