小檗碱肠溶微囊的研制  

Preparation of berberine enteric microcapsules

作　　者：王静[1] 方信[1] 王璐[1] 孙占鳌[1] 刘家国[1] 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《中国兽医学报》2014年第8期1328-1331,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31172355);公益性行业(农业)科研专项资助项目(201303040-4);国家大学生创新性实验计划资助项目(201210307052);山东省科技发展计划资助项目(2012GGC15003);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为提高小檗碱生物利用度,并改善其对动物的适口性,采用醇提法从黄连中提取出小檗碱成分,以液中干燥法制备小檗碱成分肠溶微囊,并通过单因素试验对影响微囊形成的几个关键条件进行筛选。结果显示,小檗碱成分肠溶微囊制备的最佳工艺条件为:小檗碱∶羟丙基甲基纤维素邻苯二甲酸酯(HPMCP)=1∶7,HPMCP∶丙酮乙醇混合液=1∶20,丙酮乙醇混合液∶液体石蜡=1∶5,司班80∶液体石蜡=6∶100。以此工艺制备3批微囊,其平均包封率为93.32%,微囊外观规整,呈现均匀的流动性和分散性,粒径大小在5～55μm,平均粒径为30.54μm;微囊在人工胃液中2h内溶出少于2%,而在人工肠液中2h释放达到97.343%,几乎达到完全释放,符合中华人民共和国药典的要求。To enhance bioavailability and improve its palatability of berberine, alcohol formulation was used to extract berberine from Rhizoma coptidis ,then the method of drying in fluid was used to prepare the berberine enteric microcapsule. The single factor experiments was used to observe the several key conditions affecting the formation of the microcapsule. The optimum conditions for the preparation of berherine enteric microcapsules are as follows., herherine ： HPMCP is 1 ： 7, HPMCP ： the mixture liquid of acetone and alcohol is 1 ： 20,the mixture liquid of acetone and al- cohol ： liquid paraffin is 1 ： 5,Span 80 ＇ liquid paraffin is 6 ： 100. The microcapsules prepared has uniform appearance,with an average particle size of 30.54 ,urn ranging 5-55μm,and with an aver age encapsulation efficiency of 93. 32%. The dissolution rate of microcapsule in artificial gastric juice for 2 h was less than 2% ,in the artificial intestinal juice for 2 h released 97.34a% ,complied with the requirement of Chinese pharmacopoeia.

关 键 词：小檗碱 肠溶微囊 液中干燥法 

分 类 号：S853.7[农业科学—临床兽医学]