中药地翁颗粒对小鼠脾淋巴细胞功能的影响  

Effect of Diweng Keli on immune function of mouse spleen lymphocyte

作　　者：夏方[1] 冷春青 付运星 吴永丽[1] 郭勋[1] 董海兵[1] 张立艳[1] 白换力[1] 吴帅成[1] 韦旭斌[1] 伊鹏霏[1] 付本懂[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2014年第8期1341-1344,1348,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372470)

摘　　要：为观察中药地翁颗粒在体外对小鼠脾淋巴细胞功能的影响,设置高(100mg/L)、中(10mg/L)、低(1mg/L)3个剂量组,采用3 H法进行刀豆蛋白A(ConA)、脂多糖(LPS)诱导脾淋巴细胞增殖试验,ELISA方法检测ConA或金黄色葡萄球菌Cown株灭活菌体(SAC)刺激的脾淋巴细胞上清液IL-2、IL-6、IL-10、IL-12、IFN-γ和TNF-α的含量。结果显示,在安全浓度范围内,地翁颗粒能够明显促进T淋巴细胞和B淋巴细胞增殖;能够促进ConA刺激的淋巴细胞分泌INF-γ;能够促进SAC刺激的淋巴细胞分泌IL-12、INF-γ和IL-6,并抑制IL-10的分泌。结果表明,地翁颗粒能够有效调节小鼠脾淋巴细胞的功能,促进细胞免疫。To examine the effect of Diweng Keli on immune function of mouse spleen lymphocyte in vitro,the blastogenesis of the responder cells was quantified by a colorimetric method using MTT in 96-well microculture plates. Then,we investigated the effects of Diweng Keli on the proliferative activity of lymphocytes stimulated by concanavalin A（Con A） and lipopolysaccharide（LPS） by 3 H- thymidine and cytokine levels in the supernatant by ELISA. The results showed that Diweng Keli promotes the proliferative activity of lymphocytes stimulated by concanavalin A（Con A）and li- popolysaccharide（LPS） ,the production of IFN-）＇ of T cells and the production of IL-12,IFN-7 and IL-6 of antigen-presenting cells（APC） ,but inhibits the production of IL-10 of APC. These results indicated that Diweng Keli promotes cell-mediated immunity of mice and regulate mice immune function.

关 键 词：小鼠 地翁颗粒 中药 脾淋巴细胞 

分 类 号：S852.4[农业科学—基础兽医学] S853.7[农业科学—兽医学]