镉对PC12细胞的氧化损伤及α-硫辛酸的保护效应  

Protective effect of α-lipoic acid on cadmium-induced oxidative damage in PC12 cells

作　　者：张康保[1] 高倩[1] 张雅静[1] 王涛[1] 胡飞飞[1] 江辰阳[1] 王棋文[1,2] 袁燕[1] 刘学忠[1] 卞建春 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]毕节学院毕节试验区研究院,贵州毕节551700

出　　处：《中国兽医学报》2014年第8期1349-1352,1357,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31302058);江苏省优势学科基金资助项目

摘　　要：为研究α-硫辛酸(α-lipoid acid,α-LA)对镉致PC12细胞氧化损伤的保护效应,通过不同浓度的醋酸镉染毒PC12细胞,并用100μmol/Lα-LA联合作用,测定PC12细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果表明:与对照组相比,10μmol/L醋酸镉处理24h组细胞MDA含量和CAT活性极显著升高(P<0.01),SOD和GSH-Px活性极显著降低(P<0.01);不同浓度镉处理组细胞内MDA含量和CAT活性显著或极显著升高(P<0.05或P<0.01),10和20μmol/L组SOD和GSH-Px活性极显著降低(P<0.01)。α-LA保护组与相应染毒组相比,MDA含量和CAT活性显著降低(P<0.05),SOD和GSHPx活性有升高趋势,但组间差异不显著(P>0.05)。说明镉可致PC12细胞发生氧化应激,α-LA可以提高PC12细胞的抗氧化能力,对镉引起的氧化损伤有一定的保护作用。To investigate the protective effect of α-lipoic acid on cadmium-induced oxidative damage in PC12 cells. The PC12 cells were exposured to cadmium at different time（0,6,12,24,48 h）, dif- ferent concentrations（0,5,10, 20μmol/L）, or α-LA （100 μmol/L） with cadmium （10 μmol/L） for 24 h. Then, the activities of superoxide dismutase （ SOD）, catalase （CAT）, glutathione peroxidase （ GSH- Px）and the content of malondiadelhyde （MDA）in cells were detected. Compared to the control group,the activity of CAT and the content of MDA increased significantly（P^0.01）and the activ- ities of SOD and GSH-Px decreased significantly（P^0.01） in 10 pmol/L Cd. Meanwhile, compared to the control group,the activity of CAT and the content of MDA increased significantly（P〈0.05 or P〈 0.01）. Whereas the activities of SOD and GSH-Px decreased significantly（P〈0. 01）in 10 and 20/2mol/L Cd. When compared to the poisoning group, the activity of CAT and the content of MDA decreased with significant difference（P〈0.05） ,but the activities of SOD and GSH-Px were increased with no significant difference in α-LA treated groups （P〉0.05）. The results indicated that α-LA could increase antioxidant effects and reduce lipid peroxidation caused by cadmium.

关 键 词：镉 PC12细胞 Α-硫辛酸 氧化损伤 

分 类 号：S856.9[农业科学—临床兽医学]