不同品种猪IGFBP-3启动子区突变位点筛查及分析  

The screening and analysis of the mutation sites of IGFBP-3 gene promoter sequence in different pig breeds

作　　者：吴庆燕[1] 贾泓瑶[2] 程云云[1] 张昊龙[2] 郭炜彤 石惠[1] 李思明[1] 郝林琳[1] 刘松财[1,3] 卢可[4] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学临床医学院,吉林长春130021 [3]吉林省五星动物保健药厂,吉林长春130062 [4]吉林大学化学学院,吉林长春130012

出　　处：《中国兽医学报》2014年第11期1800-1804,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31101781;31072102);高等学校博士学科点专项科研基金项目(20110061110081)

摘　　要：以军牧1号白猪、大白猪、东北野猪、巴马香猪和西藏小型猪为研究对象,通过PCR扩增5个品种猪IGFBP-3基因5′调控区序列(约2 000bp)并进行测序分析,来研究不同品种猪IGFBP-3基因启动子区序列的差异。同源性比对分析发现5个品种猪IGFBP-3基因5′调控区存在约50处突变;启动子预测发现在-185bp附近为核心启动子区的几率最大;突变共导致9处转录因子结合位点发生改变,其或可造成不同品种猪IGFBP-3表达量的差异,进而影响不同品种猪的体型大小。In order to explore the variance of IGFBP-3 among different pig breeds, the 5＇-end of IG- FBP 3 gene sequence （about 2 000 bp） was amplified by PCR and analyzed, the Junmu No. 1 white pig, the large white pig, the Northeast wild pig, the special Chinese Bama Xiang pig and the Tibetan mini-pig were taken as the objects of the study. The homology alignments indicated about 50 mutations in the pig inter-species. The core promoter regions have the greatest existence in the around of --185 bp. The mutations caused 9 changes of the transcription factor binding sites. These differences may cause different expression of IGFBP-3 among different pig breeds,thereby affect- ing the body size between the large and mini pigs.

关 键 词：IGFBP-3 转录因子结合位点 核心启动子 

分 类 号：S852[农业科学—基础兽医学]