大鼠高浓度矽尘接触与氧化应激的研究  

Study on High Concentrations of Silica Dust Exposure Process on Rats in Oxidative Stress

作　　者：赵建磊[1,2,3] 王瑞[1] 张海东[1] 王辉[1] 张海鹏[1] 于颐光 

机构地区：[1]山东省职业卫生与职业病防治研究院,山东济南250062 [2]济南大学山东省医学科学院医学与生命科学学院 [3]胶州市铺集镇中心卫生院

出　　处：《中国辐射卫生》2014年第5期465-468,共4页Chinese Journal of Radiological Health

基　　金：国家自然科学基金资助项目(30872094);山东省科技攻关项目(2013GSF11828);济南市科技发展计划项目(201303044);山东省医药卫生科技发展计划项目(2011HD011)

摘　　要：目的目的探讨大鼠高浓度矽尘接触过程中是否存在氧化应激反应。方法选40只Wistar雄性大鼠随机分为4组,即高剂量染尘组(1000 mg/m3)、中剂量染尘组(500 mg/m3)、低剂量染尘组(100 mg/m3)和对照组,选用动式染尘装置每天染尘2小时。染尘49天后处死大鼠,测定血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)活性及丙二醛(MDA)、还原性谷胱甘肽(GSH)含量;取肺组织HE染色光镜下观察病理形态的改变。结果长时间、高浓度矽尘接触降低血清SOD、TAC、GSH、T-AOC水平,同时MDA含量升高,与对照组比较,差异有统计学意义(P<0.05),病理学检查出现炎症性特征变化。结论大鼠高浓度矽尘接触过程中体内可能存在氧化系统与抗氧化系统的失衡,氧化应激反应可能是矽尘接触早期症状之一。Objective To investigate the existence of oxidative stress in rats with high concentrations of silica dust exposure process. Methods Elections of 40 SD rats were randomly divided into four groups, namely high dose of dust exposure group （1000 mg/m3） , middle dose of dust exposure group （500 mg/m3） , low dose of dust exposure group （100 mg/m3） and the control group, Dynamic stained dust 2h every day. The rats were sacrificed after staining dust 49d, serum of superoxide dismutase （SOD）, catalase （CAT）, total antioxidant capacity （T-AOC） activity ,malondialdehyde （MDA）andreduced glutathione （GSH） levels were observed. Meanwhile,lung tissue pathological changes was observed by light microscopy morpholo- gy. Results The long, high concentrations of silica dust exposure reduced SOD, TAC, T - AOC activity and GSH contens in serum, while increasing MDA content, compared with the control group, there was a significant statistical difference （ P 〈 0. 05）. Pathological examination showed inflammation lesions. Conclusion Unbalance between oxidation system and antioxidation system in vivo possible exist in high concentration silica exposure process. Oxidative stress may be one of the early symptoms of silica dust exoosure.

关 键 词：大鼠 高浓度矽尘 氧化应激 

分 类 号：R135.2[医药卫生—劳动卫生]