SNP和SSR标记在小麦Heyne×Lakin重组自交系群体中的偏分离分析  

Genetic Analysis of Segregation Distortion of SNP and SSR Markers in Wheat RIL Population from the Cross Heyne×Lakin

作　　者：王慧兰[1,2] 刘红侠[3] 王娜[1] 赵朋[1] 李春莲[1] 王中华[1] 

机构地区：[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学农学院,陕西杨凌712100 [2]山西省农产品质量安全检验监测中心,山西太原030025 [3]西北农林科技大学成教学院,陕西杨凌712100

出　　处：《麦类作物学报》2014年第9期1205-1209,共5页Journal of Triticeae Crops

基　　金：教育部留学回国人员基金项目(K308021401);2013年西北农林科技大学唐仲英育种基金项目

摘　　要：为探究小麦偏分离规律及给小麦农艺性状的QTL定位研究提供相关信息,对普通小麦Heyne×Lakin的145个重组自交系的2 241个标记基因型(包括2 071个SNP标记和170个SSR标记)进行连锁作图及偏分离分析,检测SNP和SSR标记偏分离的热点区域(SDR)。结果表明,共有2 197个标记(占总标记数的98.0%)定位在覆盖小麦21条染色体的42个连锁群中,连锁遗传图谱的总长度为2 265.9cM,标记间的平均遗传距离为1.03cM。在连锁遗传图谱中,共有230个标记位点表现偏分离,占总标记数的10.5%。其中164个标记偏向父本Lakin,占偏分离标记数的71.3%;66个标记偏向母本Heyne,占偏分离标记数的28.7%。检测到11个SDRs,分别位于2A、2B、2D、3B、5D、6B和7A染色体上,其中有5个SDRs偏向母本Heyne,6个SDRs偏向父本Lakin,推测这些偏分离热点区域的形成可能与配子体选择有关。In order to detect the hot regions of marker segregation distortion and provide theoretical basis for the research on wheat segregation distortion and QTL mapping, 2 241 markers （including 2 071 SNP markers and 170 SSR markers） were used for genotyping of 145 RILs from common wheat cross Heyne×Lakin. A high density linkage map was constructed and segregation distortion analysis of SNP and SSR markers was conducted. The results showed that a total of 2 197 markers, accounting for 98.0% of total markers, were mapped on 42 linkage groups covering all the 21 wheat chromosomes. The distance of this genetic map is 2 265.9 cM with average marker interval 1.03 cM. There were 230 markers, accounting for 10.5% of total markers in the map showed segregation distortion. Among them, 164 distorted markers, accounting for 71.3%, were bias to male parent Lakin and 66 distorted markers, accounting for 28.7%, were skew to female parent Heyne. 11 segregation distortion regions （SDRs） were detected on chromosomes 2A, 2B, 2D, 3B, 5D, 6B and 7A. 5 SDRs were distorted to Heyne, while 6 SDRs were skewed to Lakin, which inferred that segregation distortion of molecular markers could be the result of gametic selection in the cross of Heyne×Lakin.

关 键 词：小麦 重组自交系 分子标记 遗传图谱 偏分离 

分 类 号：S512.1[农业科学—作物学] S330