转ABP9基因冬小麦的抗旱性评价  

Evaluation of Drought Resistance in ABP9-transgenic Winter Wheat Lines

作　　者：王炜[1] 罗影[2] 王剑虹[3] 裴怀弟[1] 陈玉梁[1] 叶春雷[1] 罗俊杰[1] 杨随庄[4] 

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]甘肃农业大学资源与环境学院,甘肃兰州730070 [3]兰州职业技术学院生物工程系,甘肃兰州730070 [4]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《麦类作物学报》2014年第9期1217-1224,共8页Journal of Triticeae Crops

基　　金：国家转基因生物新品种培育重大专项(2009ZX08005-013B);甘肃省科技支撑项目(1104NKCA073);甘肃省农业科学院农业科技创新专项(2009GAAS15);甘肃省省级财政农业科技创新项目(GNCX-2013-47)

摘　　要：为了解转ABP9基因冬小麦材料的抗旱性,在雨养和灌溉条件下,对32份转ABP9冬小麦株系及其受体亲本石4185的主要农艺性状和抽穗期部分生理指标进行了测定,并采用隶属函数值法和抗旱性度量(D)值法对其抗旱性进行了综合评价,利用灰色关联度法对相关抗旱性状与抗旱指数之间的关联度进行了分析。结果表明,采用隶属函数值法和D值法评价时,分别有6和10份转基因小麦材料的抗旱性较受体亲本石4185增强。各农艺性状和生理指标依照与抗旱指数的关联度从高到低依次为单穗粒重、结实小穗数、千粒重、穗粒数、叶绿素含量、POD活性、MDA含量、SOD活性、株高、有效分蘖。因此,在对转ABP9基因抗旱小麦进行鉴定筛选时,应主要以单穗粒重、结实小穗数、千粒重、穗粒数等农艺性状作为参考,适当考虑生理指标的影响;由于不同指标或性状对小麦抗旱性的贡献不同,采用抗旱性度量值较平均隶属函数值进行抗旱性综合评价更为可靠。A total of 11 traits, including 7 agronomical traits and 4 biochemical traits were measured in 32 ABP9 transgenic winter wheat lines and corresponding receptor parent Shi 4185 grown in rain fed （drought stress） and well watered environment, the drought resistance of these materials were evaluated comprehensively by using the methods of subordinate function and drought resistance measure value （Dvalue）, and the correlation degree between these traits and drought resistance index was analyzed and evaluated by using gray relational grade analysis method.The results showed that the drought resistance of 6 and 10 transgenic wheat lines exceeded their parent Shi 4185 when evaluated by using the methods of subordinate function and D value, respectively; the results of gray relational grade analysis indicated that the correlation degree between these traits and drought resistance index were ranked from high to low as follows： single spike weight, spikelet numbers per ear, 1 000 kernel weight, number of kernel per spike, chlorophyll content, POD activity, MDA content, SOD activity, plant height, effective tillers.In conclusion, the agronomic traits such as single spike weight, spikelet numbers per ear, 1 000 kernel weight, number of kernel per spike could be used as the main drought index in determination and selection of drought resistant ABP9 transgenic wheat lines, and the biochemical traits may be taken as reference; given different traits index was not always contribute to drought resistance equally in wheat, drought resistance measure value （Dvalue） was more accurate and reliable than the methods of average subordinate function in comprehensive evaluation of drought resistance in crops.

关 键 词：小麦 ABP9基因 抗旱性 隶属函数 灰色关联度 

分 类 号：S512.1[农业科学—作物学] S330