苏云金芽孢杆菌高效杀蚊菌株BRC-ZLL13的鉴定  

Identification and Characterization of Bacillus thuringiensis Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes

作　　者：刘昭霞[1] 李明伟[1] 吴松青[1,2] 叶文惠 余雄泽 林秋秋[1] 关雄[1] 张灵玲[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [2]福建农林大学林学院,福州350002

出　　处：《基因组学与应用生物学》2014年第5期1031-1038,共8页Genomics and Applied Biology

基　　金：国家863项目(2011AA10A203);教育厅高校领军人才(k8012012a);国家卫生计生委共建科学研究基金--福建省卫生联合攻关计划(WKJ-FJ-25);国家自然科学基金(31301724);福建省科技计划重点项目(2013N0003);福建省自然科学基金(2013J01079);福建省高校杰出青年科研人才培育计划(JA12092);福建省新世纪人才支持计划(K80MKTO3a)共同资助

摘　　要：苏云金芽孢杆菌BRC-ZLL13是一株从白玉兰叶片中分离的高效杀蚊菌株。它对埃及伊蚊、白纹伊蚊和致倦库蚊的毒力均高于国际标准菌株以色列亚种IPS82,其生长速度也明显快于后者。本实验对该菌株毒力、血清型、生理生化反应、药敏实验、质粒、杀蚊基因和晶体蛋白等方面的生物学特性进行了研究。结果显示,BRC-ZLL13与对照菌株IPS82同属于以色列亚种,均含有cyt1、cyt2、cry4A、cry4B、cry10和cry11等杀蚊基因,二者在生理生化反应、药敏实验和晶体蛋白图谱等生物学特性上也表现出极高的相似性。另一方面,前者比后者少2条质粒带,这可能是导致BRC-ZLL13生长速度较快的主要原因。综上所述,笔者认为该菌株在公共卫生和生物防治领域具有开发和应用潜力。Bacillus thuringiensis strain BRC-ZLL13 was isolated from leaves of Magnolia aenudate. It was char- acterized based on its insecticidal activity againstAedes aegypti, Aedes albopictus, and Culex quinquefasciatus lar- vae, flagellar antigen serology, biochemical reactions, antibiotic sensitivity, plasmid profile and crystal protein pro- file as well as PCR analysis using novel general and specific primers for cry and cyt genes analysis. It showed more than twofold higher mosquitocidal activity against A. aegypti, A. albopictus and C. quinquefasciatus larvae than B. thuringiensis subsp, israelensis （IPS82） and displayed high similarities with the IPS82 with regard to pro- tein and the presence of mosquitocidal genes, but had different plasmid profiles and higher growth rate. These re- sults suggested that the loss ofplasmid might be contribute to high growth rate of this strain, and indicated a poten- tial for developing this strain for application in the fields of public hygiene and biological control.

关 键 词：苏云金芽孢杆菌 杀蚊 生物学特性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]