a溶血素纳米孔的研究进展和应用  

The Research Progress and Application of a-Hemolysin Nanopore

作　　者：张春萍[1] 

机构地区：[1]西北大学化学与材料科学学院,西安710069

出　　处：《基因组学与应用生物学》2014年第5期1114-1118,共5页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31100107)资助

摘　　要：近年来,随着各学科的交叉融汇,以膜蛋白纳米孔为基础元件的纳米通道单分子检测技术显现出诱人的前景,尤其是以a-溶血素纳米孔为核心元件的检测器件在单分子分析、单分子化学反应研究和DNA测序等方面具有独特的价值而受到重视。本文结合本课题组的研究内容,对以a-溶血素纳米孔为代表的膜蛋白单分子检测的研究进展、功能化制备及组装机理进行了总结,主要概述了膜蛋白作为单分子传感器件在基因测序方面的应用发展状况、可能存在问题以及未来发展前景。In recent years, with the cross of multi-disciplinary, single molecule detection technology based on the nanopore membrane protein has shown an attractive prospect. The detection devices using α- hemolysin nanopore as core component have been paid more and more attention because of its unique value in single molecule analysis, single molecule chemical reaction and DNA sequencing. In this review, the progress of membrane protein in single molecule detection, the preparation of functional nanopore and the assembly mechanism of nanopore with α-hemolysin as example are summarized. The application, possible problems and future development prospect in DNA sequencing using membrane protein as single molecular sensors are mainly outlined.

关 键 词：a溶血素 纳米孔 单分子检测 

分 类 号：Q81[生物学—生物工程] TB383.1[一般工业技术—材料科学与工程]