Effect of Long-Term in Vitro Sub-culturing on Quality Degeneration of Sweet Potato Varieties： Morpho- Anatomic Assessment and Simple Sequence Repeats Analysis  

作　　者：Mihiretu Cherinet Hundayehu Elsa Du Toit Sunette Laurie Martin Steyn Ria Greyling Nokuthula Myeza 

机构地区：[1]Department of Plant Production and Soil Science, University of Pretoria, Lynwood Road, Pretoria 0002, South Africa [2]Agricultural Research Council-Roodeplaat Vegetable and Ornamental Plant Institute (ARC-VOPI), Private Bag X293, Pretoria0001, South Africa

出　　处：《Journal of Agricultural Science and Technology(A)》2014年第10期811-821,共11页农业科学与技术（A）

摘　　要：The aim of this study was to assess the effect of long-term in vitro sub-culturing on the varietal degeneration of three sweet potato varieties, namely, Monate, Mokone and Ndou which were sub-cultured for 32, 23 and 12 generations, respectively. Each generation was cultured in a media which is made from 4.43 g/L Murashige and Skoog （MS）, 30 g/L sucrose and 2 g/L gelrite, respectively, and grown under 16 h light and 8 h dark photoperiod for 30 d. For each generation, 45 plantlets were acclimatized for two months in a glasshouse. Data on in vitro growth performance and 11 morphological characteristics during acclimatization were recorded. Early root and shoot formation was observed after the 27th and 21st sub-cultured generations of Monate and Mokone, respectively. During acclimatization, plantlets from the same variety showed differences in morphological traits such as leaf colour, abaxial leaf pigmentation, vine pigmentation, petiole pigmentation, leaf wrinkling and flowering. However, the rate of these morphological differences is random and irrespective to increase in sub-culturing. Therefore, to understand the genetic base of these morphological variability, two plantlets from each variety were subjected to genetic analysis by using five simple sequence repeat （SSR） primers （IB-242, IB-318, IB-255F, 1B-248 and IB-255）. Although SSR loci IB-255F and IB-318 could distinguish between the three varieties, there were no allelic polymorphisms detected in plantlets from the same varieties. Therefore, long-term sub-culturing do not leads to quality degeneration in the three sweet potato varieties.

关 键 词：MICRO-PROPAGATION ORGANOGENESIS SUB-CULTURE true-to-type morphology polymorphism. 

分 类 号：S722[农业科学—林木遗传育种] S531.023[农业科学—林学]