Modification of Campylobacterjejuni Broiler Colonization by a Feed Additive Composed of Encapsulated Organic Acids and Essential Oils  

作　　者：Alexandre Thibodeau Philippe Fravalo Robert Gauthier Evelyne Guevremont Nadia Bergeron Sylvette Laurent-Lewandowski Sylvaln Quessy Martine Boulianne Ann Letellier 

机构地区：[1]Department of Pathology and Microbiology, University of Montreal, Saint-Hyacinthe, Quebec J2S 2M2, Canada [2]Food Research and Development Centre, Agriculture and Agri-Food Canada, Saint-Hyacinthe, Quebec J2S 8E3, Canada [3]JEFO Nutrition Inc., Saint-Hyacinthe, Quebec J2S 7B6, Canada

出　　处：《Journal of Agricultural Science and Technology(A)》2014年第10期853-864,共12页农业科学与技术（A）

摘　　要：The objective of this study was to evaluate the effect of a novel feed additive on chicken intestinal colonization and carcass contamination by Campylobacterjejuni. The feed additive was composed of microencapsulated organic acids and essential oils （OA/EO）. The feed additive tested was provided by Jefo Nutrition Inc., St-Hyacinthe, Quebec, Canada. Day-old birds were separated into two rooms and subdivided into two groups. Chicken were fed with OA/EO or not fed with OA/EO until they reached 35 d of age. At 14 d of age, chickens received an oral suspension of two well characterized C. jejuni strains, depending on the room they were housed in. The levels of C. jejuni were periodically monitored in the caecum and on the carcasses. C. jejuni colonization was further characterized by the use of high-resolution melt analysis of the C. jejuniflaA gene （HRM-flaA）. The effect of the feed additive was strain-dependent. In room two, the feed additive had no effect on the caecal counts. In room one, at 35 d of age, caecal C. jejuni counts were higher with OA/EO, as opposed to carcasses counts which were lower in the treated group. The HRM-flaA analysis showed that an amplification profile was predominant in birds fed with OA/EO at 35 d of age in room one, suggesting the selection of a C. jejuni strain. In conclusion, the OA/EO seemed to be effective to reduce C. jejuni levels but this effect appeared strain dependent.

关 键 词：C. jejuni control feed additive HRM organic acids and essential oils chicken colonization. 

分 类 号：S816.7[农业科学—饲料科学] S858.315.1[农业科学—畜牧学]