西瓜抗枯萎病育种的分子辅助选择  

Study on Molecular Marker Assisted Selection for Fusarium Wilt Resistance Breeding in Watermelon

作　　者：王吉明[1] 尚建立[1] 董亚玲[1] 薛盈盈[1] 马双武[1] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009

出　　处：《中国农学通报》2015年第7期66-71,共6页Chinese Agricultural Science Bulletin

基　　金："十二五"农村领域国家科技计划课题"西瓜种质资源创新利用"(2013BAD01B04-21)

摘　　要：利用中国国内公开发表的西瓜抗枯萎病生理小种1基因紧密连锁标记4451_fon,首次对抗枯萎病育种创新的二倍体和四倍体种质进行分子鉴定。结果表明,全部二倍体单系和除编号D16、D17外的四倍体单系均表现为抗病基因型,判定为抗病材料,与人工接种鉴定结果完全一致;分子鉴定还表明,这些抗病单系为抗病纯合体,在以后的自交纯化过程中抗性基因不再分离和丢失,因此不必继续对其自交后代进行抗性选择。研究结果认为d CAPS标记4451_fon可以作为西瓜抗枯萎病分子辅助选择(molecular marker assisted selection,MAS)的重要工具,能够实现提高选择效率、加速育种进程的目的。In this report, study on molecular identification of Fusarium wilt resistance for diploid andtetraploid watermelon breeding lines using d CAPS marker＂4451_fon＂which published in 2013 was carriedout. The results showed that all diploid lines and tetraploid lines except No.D16 and D17 were Fusarium wiltresistant genetype material, could be identified as having Fusarium wilt resistance, and the result was inaccordance with artificial inoculation identification. Molecular identification results also showed that thoseresistant lines were homozygote, so Fusarium wilt resistant gene would not separate when lines self-crossed,and there was no necessary carry out single resistant plant selection in their progeny population. The finalconclusion were that d CAPS Marker＂4451_fon＂could be applied to molecular marker assisted selection for Fusarium wilt resistance breeding in watermelon to increase breeding selection efficiency and enhancebreeding progress.

关 键 词：西瓜 枯萎病 分子辅助选择 

分 类 号：S651[农业科学—果树学]