中药远志对锰中毒小鼠微管相关蛋白表达的影响  

Effects of polygala on the neurogenesis of manganese poisoned mice

作　　者：井学超 覃丽[1] 杨伯宁[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021

出　　处：《中华劳动卫生职业病杂志》2015年第3期207-210,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广西自然科学基金资助（2010GXNSFA013157）

摘　　要：目的 探讨远志对锰中毒小鼠学习记忆和微管相关蛋白表达的影响.方法 成年雌性昆明小鼠60只,随机分为正常对照组（生理盐水）、锰中毒组、锰中毒＋远志高剂量组（10.0 g/kg）、锰中毒＋远志中剂量组（5.0 g/kg）、锰中毒＋远志低剂量组（2.5 g/kg）,每组12只,采用腹腔注射氯化锰（MnCl215 mg/kg）染毒.Morris水迷宫实验检测小鼠学习记忆能力,免疫荧光染色检测小鼠侧脑室室管膜下区（SVZ）和海马齿状回颗粒下区（SGZ）内微管相关蛋白Doublecortin（DCX）的表达.结果 与锰中毒组比较,远志高、中、低剂量组逃避潜伏期明显缩短,差异有统计学意义（P＜0.05）远志高、中、低剂量组穿越平台次数比锰中毒组明显增多,差异有统计学意义（P＜0.05）.远志高、中、低剂量组SVZ和SGZ的DCX表达明显多于锰中毒组,差异有统计学意义（P＜0.05）.结论 远志干预可改善锰中毒小鼠的学习记忆能力,其机制可能与其促进脑内DCX的表达及神经发生有关.Objective To investigate the effects of polygala on leaning and memory and the expression of Microtubule associated protein on manganese poisoned mice.Methods 60 female Kunming mice were randomly and equally divided into 5 group.They are normal control group （CG）,manganese poisoned group （MG）,manganese poisoned with polygala high dose group （MHG）,manganese poisoned with polygala middle dose group （MMG）,manganese poisoned with polygala low dose group （MLG）.The model of manganese poisoned mice was prepared of the way of intraperitoneal injection of manganese chloride （MnCl2 15 mg/kg）,the spatial learning and memory ability was tested by Morris water maze,the Doublecortin （DCX） was tested by the way of immunofluorescent staining in the SVZ and SGZ.Result In the navigation test,compared with MG,the escape latency of MHG,MMG and MLG were significantly decreased （P〈0.05）,in space exploration experiments,MHG,MMG,MLG compared with MG,the number increased significantly across platforms （P〈0.05）.compared with MG,the DCX expression of MHG,MMG and MLG were significantly increased （P〈0.05）.Conclution The leaning and memory ability of manganese poisoned mice can be improved by the polygala,and the mechanism may be related to promote the expression of DCX and neurogenesis in the brain.

关 键 词：植物 用药(远志) 锰 微管相关蛋白蛋类 Plans medicinal(polygala) 

分 类 号：R285.5[医药卫生—中药学]