猪胰岛移植于大鼠肝包膜下人工囊腔治疗糖尿病的研究  

Pig pancreas islets transplanted in an artificial cyst under capsule of liver for diabetic mellitus in rats

作　　者：徐兵[1] 吴林岚[2] 杨晓梅 黄素钦[1] 吴开木[2] 

机构地区：[1]福建医科大学孟超肝胆医院肝病研究所,福州350025 [2]福建省第二人民医院检验科 [3]福州市菁华崇辉医院肝内科

出　　处：《中华器官移植杂志》2015年第2期108-112,共5页Chinese Journal of Organ Transplantation

基　　金：福州市科技局项目（2013-125-6）

摘　　要：目的建立一种新的免疫隔离法用于将猪胰岛移植于大鼠肝包膜下人工囊腔，并观察其治疗糖尿病大鼠的效果。方法用链脲霉素诱导建立大鼠糖尿病模型。用V型胶原酶消化猪胰腺，梯度离心法分离纯化猪胰岛。检测纯化猪胰岛的胰岛素刺激指数。取18只糖尿病大鼠和6只正常大鼠，手术暴露大鼠肝脏，从肝叶一侧边缘切开并分离肝包膜，将人工囊腔（纤维素酯透析袋，袋内置一中空纤维球，袋两端用线扎紧）两端从肝包膜切口分别塞入两边肝包膜下，囊腔两端连线从肝叶对侧边缘肝包膜开孔穿出并相互连接，绑于肝叶；使囊腔平铺在肝实质表面，中段含纤维球部分位于皮下肝包膜切口问。将含胰岛（4000IEQ）的胶原溶液（pH7．4）注入糖尿病大鼠肝包膜下人工囊腔，作为实验组（n=12）；将不含胰岛的胶原溶液注入糖尿病大鼠（糖尿病对照组，n=6）或正常大鼠肝包膜下人工囊腔（正常对照组，n=6）。缝合切口（囊腔中胶原溶液变成胶原凝胶）。首次移植后8周，实验组各大鼠再经皮向肝包膜下人工囊腔注射胰岛（2000IEQ）悬液1次。各组大鼠每周断尾取血检测空腹血糖值。结果纯化猪胰岛的胰岛素刺激指数为2．2±0．2。首次移植后1～8周，实验组大鼠各时间点空腹血糖值明显低于糖尿病对照组大鼠（P〈0．01）。首次移植后13周，肉眼观察大鼠肝包膜下人工囊腔周围，未见明显纤维组织增生。结论纤维素酯透析袋植入肝包膜下13周对周围组织没有明显刺激。猪胰岛在大鼠肝包膜下人工囊腔中可受到免疫隔离，并正常生长，发挥降血糖功能。可经皮注射将猪胰岛移植于大鼠肝包膜下人工囊腔。Objective To develop a new method of immune isolation for transplanting pig islets to rat to observe the effects on diabetic rats. Method Diabetes of rat was induced by streptozotocin. Pancreas of pig was digested with type V collagenase. The pig islets were purified by density gradient centrifugation. Insulin stimulation index of the purified pig islets was evaluated. Liver of the 18 diabetic rats and 6 normal rats was exposed by operation. Capsule at one edge of the liver lobe was cut apart and separated from liver parenchyma on both sides of the lobe. An artificial cyst was constructed by a cellulose ester （CE） dialysis bag with a ball of hollow fibers in the bag, and each end of the bag was closed by a thread. Each end of the bag was stuffed into the subcapsule of each side of the liver lobe. The thread at each end of the bag was passed through a hole of the capsule at other edge of the lobe and linked to each other around the lobe to hold the artificial cyst on the liver parenchyma. Middle part of the cyst （with the ball of hollow fibers in） was put between the cuts of capsule and under the skin. The Collagen solution （pH 7. 4） with pig islets （4000 IEQ） was injected into the subcapsule artificial cyst of 12 diabetic rats as experiment group. The collagen solution without pig islets was injected into the subcapsule artificial cyst of 6 diabetic rats and 6 normal rats as diabetes control group and normal control group respectively. The cut was sutured. The collagen solution became a collagen gel in the artificial cysts. At 8th week of the initial transplantation, the solution with pig islets （2000 IEQ） was injected percutaneously again into the subcapsule artificial cyst of each rat of the experiment group. Rats of the three groups were raised as usual, and the levels of fasting blood glucose were measured regularly. Result The insulin stimulation index of the purified pig islets was 2. 2 ± 0. 2. At 1st to 8th week of the initial transplantation, the levels of fasting blood glucose in the

关 键 词：猪 胰岛 移植 大鼠 肝 包膜下 免疫隔离 

分 类 号：R657.5[医药卫生—外科学]