两栖类壶菌病3种PCR鉴定方法与比较  

Three Kinds of Identification Method and Comparison about Amphibian Chytridiomycosis

作　　者：李斌[1] 黄艳[1] 王宇[1] 邵晨[1] 

机构地区：[1]浙江师范大学生态研究所,浙江金华321004

出　　处：《安徽农业科学》2015年第11期32-34,49,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31400472);浙江省自然科学基金项目(LQ14C040001);浙江师范大学博士科研启动基金项目(ZC304013020)

摘　　要：壶菌病是一种新型的由蛙壶菌感染所引起的两栖动物传染疾病,其快速的传染性和广泛的暴发性导致两栖类物种的大面积灭绝,对全球两栖动物的生存产生了巨大影响。介绍了3种鉴定蛙壶菌的PCR方法:常规PCR、巢式PCR和实时定量PCR,并对这3种方法进行分析与比较。Chytridiomycosis is an emerging infectious diseases caused by the Batraehochytrium dendrobatidis （Bd）. Its rapid infectivity and extensive explosion leads to amphibian species extinction in large area and that has a huge influence on amphibians live on the global. Three identification methods of PCR assay were introduced ： single-round PCR, nested PCR and real-time TaqMan PCR, the comparison and analysis of these three methods were conducted.

关 键 词：壶菌病 蛙壶菌 两栖动物 PCR 

分 类 号：S188[农业科学—农业基础科学]