不同培养基条件对红景天花药愈伤组织生长量的影响  

Effect of Different Culture Medium on the Plant Tissue Growth of Rhodiola sachalinensis Anther Callus

作　　者：盖玉红[1] 魏健[2] 

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]长春师范大学生命科学学院,吉林长春130032

出　　处：《黑龙江农业科学》2015年第4期10-12,37,共4页Heilongjiang Agricultural Sciences

摘　　要：为进一步开发利用红景天资源,以红景天花药诱导的愈伤组织为材料,研究了不同培养基、不同种类和浓度的生长素和细胞分裂素对花药愈伤组织生长量的影响。结果表明:培养基MS和NB对花药愈伤组织生长量的影响无显著差异;低浓度的生长素对红景天花药愈伤组织生长量有利,2,4-D对愈伤组织生长的影响略高于NAA。高浓度的6-BA利于在景天花药愈伤组织生长,在含有相同浓度NAA的培养基中,6-BA比KT适合花药愈伤组织生长。In order to further develop and utilize the resource of Rhodiola sachalinensis,taking its callus induction anther as materials,the effect of different medium,different type and concentration of plant hormone and cytokin on anther callus growth were studied.The results showed that on anther callus growth had no significant difference,the lower concentration of IAA could promote the grouth of anther callus,and the effect of 2,4-D was higher than that of NAA.The higher concentration of 6-BA could promote the qrowth of anther callus.With the same basal medium,6-BA was more suitable for the growth of anther callus than KT.

关 键 词：红景天花药愈伤组织 生长量 培养基 生长素 细胞分裂素 

分 类 号：S567.239[农业科学—中草药栽培]