低水平1,3-丁二烯职业接触人群遗传损伤及其对DNA修复能力影响研究  

Study on genetic damage and DNA repair capacity among workers exposed to low level of 1,3-butadiene

作　　者：李忠生[1] 刘楠[2] 关维俊[2] 孟会林[1] 崔涛[1] 李斌[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]河北联合大学公共卫生学院,河北省煤矿卫生与安全实验室,河北唐山063000

出　　处：《中国职业医学》2015年第1期7-11,共5页China Occupational Medicine

基　　金：国家自然科学基金(30471471)

摘　　要：目的探讨职业性低水平接触1,3-丁二烯（BD）所致周围血淋巴细胞遗传损伤与DNA修复能力的相关性。方法采用横断面调查方法,以某石化企业60名BD作业人员为接触组,按照年龄和工龄1∶1匹配,以该企业无职业性接触BD的60名工作人员作为对照组。采用直接进样-气相色谱法测定工作场所空气中BD水平;采用胞质分裂阻滞微核试验和碱性彗星试验评价2组人群周围血淋巴细胞染色体和DNA损伤水平,采用博莱霉素（BLM）诱变剂敏感性试验评价周围血淋巴细胞对BLM所致DNA损伤的修复能力。结果接触组人群工作场所空气中BD的短时间接触浓度为0.59～2.76（1.80±0.20）mg/m3,对照组工作场所空气中未检出BD。接触组人群周围血双核淋巴细胞微核率、核质桥率、核芽率和Olive尾距均高于对照组[（1.81±0.96）‰vs（1.02±0.68）‰,1.00‰（0.00～3.00）‰vs 0.00‰（0.00～1.00）‰,3.00‰（1.00～5.00）‰vs 1.00‰（0.00～3.00）‰,5.25（3.96～5.73）μm vs 2.38（1.35～3.88）μm,P〈0.01]。接触组人群周围血淋巴细胞平均每细胞染色体断裂率（b/c率）高于对照组[（1.06±0.41）%vs（0.85±0.36）%,P〈0.05]。对照组人群b/c率与微核率呈中度正相关[Spearman相关系数（rS）=0.542,P〈0.01],但均与核质桥率、核芽率和Olive尾距不相关（rS分别为0.213、0.273和-0.120,P〉0.05）;而接触组人群b/c率与微核率、核质桥率、核芽率均呈中度正相关（rS分别为0.730、0.515和0.660,P〈0.01）,但与Olive尾距不相关（rS=-0.120,P〉0.05）。结论低水平BD可能导致职业接触人群周围血淋巴细胞遗传物质损伤,其与DNA修复能力可能存在一定的相关性。Objective To explore the correlation between DNA repair capacity（ DRC） and genetic damage in peripheral blood lymphocytes of workers exposed to low level of 1,3-butadiene（ BD）. Methods With a cross-sectional study,60 workers from a petrochemical enterprise who were exposed to BD were selected as the exposure group,and 1∶ 1 matched by age and length of service,60 non-occupational BD exposure workers in the same enterprise were chosen as the control group. BD level in workplace air was measured by direct injection-gas chromatography determination method. Cytokinesisblock micronucleus（ MN） detection and alkaline comet assay were used to evaluate chromosomal and DNA damage levels in peripheral blood lymphocytes of the two groups. DRC for bleomycin（ BLM）-induced DNA damage in peripheral blood lymphocytes was measured by sensitivity test of the mutagenic agent BLM. Results The short time expose concentration of BD in the air of workplace of the exposure group was 0. 59-2. 76（ 1. 80 ± 0. 20） mg / m3,and BD was not detected in the air of workplace of the control group. The rates of MN,nucleoplasmic bridge（ NPB）,nuclear buds（ NB） and Olive tail moment（ OTM） of peripheral blood lymphocyte in the exposure group were higher than those in the control group respectively [（ 1. 81 ± 0. 96） ‰ vs（ 1. 02 ± 0. 68） ‰,1. 00‰（ 0. 00-3. 00） ‰ vs 0. 00‰（ 0. 00-1. 00） ‰,3. 00‰（ 1. 00-5. 00） ‰ vs 1. 00‰（ 0. 00-3. 00） ‰,5. 25（ 3. 96-5. 73） μm vs 2. 38（ 1. 35-3. 88） μm,P〈0. 01]. The chromatid breaks per cell（ b / c rate） of peripheral blood lymphocyte in the exposure group was significantly higher than that in the control group [（ 1. 06 ± 0. 41） % vs（ 0. 85 ± 0. 36） %,P〈0. 05]. Among the control group,the b / c rates of peripheral blood lymphocyte showed a statistical moderate positive correlation with the MN rate [Spearman correlation coefficient（ rS） was0. 542,P〈0. 01],but not related to the NPB rate,NB rate and OTM respective

关 键 词：1 3-丁二烯 职业接触 淋巴细胞 胞质分裂阻滞微核试验 彗星试验 DNA修复能力 微核 

分 类 号：R135[医药卫生—劳动卫生]