女贞子三萜类化学成分及其体外抗氧化活性的研究  

Study on the triterpenes constituents and its antioxidant activities in vitro from the fruits of Ligustrum lucidum

作　　者：江洪波[1,2] 董小萍[1] 田仁君[2] 张志强[2] 

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]四川中医药高等专科学校,四川绵阳621000

出　　处：《华西药学杂志》2015年第2期163-164,共2页West China Journal of Pharmaceutical Sciences

基　　金：四川中医药高等专科学校科研基金资助项目(14ZRYB02)

摘　　要：目的研究女贞子中三萜类成分及体外抗氧化活性。方法用甲醇浸提,反复硅胶柱色谱分离,并测定DPPH自由基的清除率。结果分离鉴定了4个三萜类化合物:3-乙酰齐敦果酸、19α-羟基-3-乙酰熊果酸、齐墩果酸、熊果酸,均具有清除DPPH自由基的能力。结论抗氧化活性为19α-羟基-3-乙酰熊果酸>3-乙酰齐敦果酸>熊果酸>齐墩果酸。OBJECTIVE To study the triterpenes from the fruits of Ligustrum lucidum and its antioxidative activity in vitro. METHODS The compounds were isolated by silica gel column chromatography repeatedly after extracting with methanol, and DPPH free radical scavenging activity was test. RESULTS Four triterpenes were isolated：3 - acetyl oleanolic, 19α - hydroxy - 3 - acetylursolic acid,oleanolic acid and ursolic acid. All of them had the activity of scavenging DPPH free radical. CONCLUSION Four triterpenes compounds show antioxidative activity, and the antioxidative activity rate is 19α -hydroxy -3 -aeetylursolic acid 〉 3 -acetyl oleanolic acid 〉 ursolic acid 〉 oleanolic acid.

关 键 词：女贞子 三萜 抗氧化 2 2-二苯基-1-苦基肼 清除率 构效关系 自由基 

分 类 号：R93[医药卫生—生药学]