rhGM-CSF和纳米银联合外用对深Ⅱ°烫伤创面上皮化的影响  

Effect of recombinant human granulocyte-macrophage colonystimulating factor combined with nano-silver on epithelization for deep burn degreen Ⅱ burn

作　　者：杨景哲[1] 温海玲[1] 耿琪瑛[1] 陈凤平[1] 冯欣姝[1] 王芳[1] 

机构地区：[1]承德医学院附属医院烧伤整形科,河北承德067000

出　　处：《华西药学杂志》2015年第2期184-186,共3页West China Journal of Pharmaceutical Sciences

基　　金：承德市科学技术研究与发展计划项目(20122164);河北省2013年医学科学研究重点课题计划指令性课题(20130024)

摘　　要：目的研究重组人粒细胞-巨噬细胞集落刺激因子(rh GM-CSF)和纳米银敷料联合外用对深Ⅱ°烫伤创面愈合过程上皮化的影响。方法用Wistar大鼠建立深Ⅱ°烫伤模型120例,均分为4组,A组用凡士林纱布覆盖、B组用纳米银敷料覆盖、C组用rh GM-CSF涂抹创面、D组用rh GM-CSF+纳米银敷料覆盖,伤后第1、4、7、10、14、21天,观察创面的病理学改变,用ELISA法测定血清中的表皮生长因子(EGF),采用RT-PCR法检测创面愈合过程中TGF-β1mRNA表达的变化。结果 4个组均在第10天出现明显的上皮化;EGF水平逐渐上升,A组上升最慢,D组最快;A、B组的TGF-β1mRNA相对表达量逐渐上升,C、D组的在第14天达到峰值,第21天下降,且D组下降幅度较大。结论 rh GM-CSF和纳米银联合外用,加速了深Ⅱ°烫伤创面愈合过程中上皮化的形成,上皮化程度优于单独应用。OBJECTIVE To observe the effect of recombinant human granulocyte - macrophage colonystimulating factor （ rhGM - CSF） combined with nano - silver as a treatment for deep degreen Ⅱ burn on epithelization. METHODS The burn model were done with Wistar rats. 120 mice were randomly divided into four groups, petrolatum treatment （group A）, nano - silver treatment （group B ）, rhGM- CSF treatment（ group C）, and rhGM -CSF combined with nano -silver treatment （group D ）. The pathological changes of wound in the four groups were observed at 1,4,7,10,14,21 days after treatment. Meanwhile the concentrations of EGF in the serum were measured by ELISA, and the levels of TGF - [31 mRNA expression were detected by RT - PCR. RESULTS All groups were epi- thelized at 10 days after treatment. EGF levels increased continuely, but group A more was slow and group D was more quick. TGF - β1 mRNA expression, group A and group B increased coutinuely, while group C and group D peaked on 14th day and then decreased. Group D decreased significantly. CONCLUSION rhGM - CSF combined with nano - silver treatment can accelerate epithelization of wound, and better than rhGM - CSF or nano - silver only.

关 键 词：外用重组人粒细胞-巨噬细胞集落刺激因子 纳米银 烧伤创面 表皮生长因子 转化生长因子β1 酶联免疫吸附法 

分 类 号：R96[医药卫生—药理学]