软坚护肝片的鉴别及大黄素的测定  

Identification of Ruanjian Hugan tablets and determination of its emodin

作　　者：郭梅红[1] 黄丽凤[1] 邱莉[1] 吴妮妮[1] 范颖[1] 孔晓龙[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《华西药学杂志》2015年第2期222-225,共4页West China Journal of Pharmaceutical Sciences

基　　金：广西科学研究与技术开发计划项目(10124008-7)

摘　　要：目的鉴别软坚护肝片并测定大黄素的含量。方法采用TLC法鉴别软坚护肝片中的丹参、黄芩、虎杖、山豆根、赤芍和三七;采用HPLC法测定片中的大黄素的含量。结果在薄层色谱图谱中可检出丹参、黄芩、虎杖、山豆根、赤芍和三七的特征斑点,重复性好;大黄素0.008-1.608μg与峰面积的线性关系良好（r=0.9999）;平均加样回收率为100.54%,RSD=1.54%（n=9）。结论所用方法简便、准确、可靠,可用于软坚护肝片的质量控制。OBJECTIVE To establish a quality standard for Ruanjian Hugan tablets. METHODS TLC was used for the identifica- tion of Salvia miltiorrhiza, Scutellariae radix, PoLygonum cuspidatum, Sophorae tonkinensis radix, Paeoniae rubra radix and Panax notog- inseng. HPLC was used to determine the content of emodin. RESULTS All characteristic spots were detected by TLC,and the repeat- ability was good. The calibration curve of emodin was linear within the range of 0. 008 - 1. 608 pog（ r= 0. 9999 ）. The average recovery was 100.54% （ RSD = l. 54% , n = 9）. CONCLUSION The method is simple, accurate and reliable then, it is able to provide some basis for the establishment of quality control methods for Ruanjian Hugan tablets.

关 键 词：软坚护肝片 薄层色谱 高效液相色谱 大黄素 鉴别 丹参 黄芪 

分 类 号：R917[医药卫生—药物分析学]