HPLC测定赤胫散中的3,3'-二甲基鞣花酸和3,3',4'-三甲基鞣花酸  

Determination of 3,3'-di-O-methyl ellagic acid and 3,3',4'-trimethyl ellagic acid in Polygonum runcinatum by HPLC

作　　者：许超飞[1] 黄家宇[1] 郝阳[1] 李莉[1] 刘青[1] 张敏[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《华西药学杂志》2015年第2期230-231,共2页West China Journal of Pharmaceutical Sciences

基　　金：贵州省中药现代化科技产业研发专项项目[黔科合中药字(2012)5034号];贵阳市科技局项目[合同(2012204)12号]

摘　　要：目的采用HPLC测定赤胫散中的3,3＇-二甲基鞣花酸和3,3＇,4＇-三甲基鞣花酸。方法色谱柱为Dikma-C18柱,流动相为甲醇-乙腈-0.1%磷酸（17∶33∶50）,流速1 m L·min^-1,检测波长248 nm,柱温30℃。结果 3,3＇-二甲基鞣花酸0.198-0.990μg（r=0.9991）、3,3＇,4＇-三甲基鞣花酸0.098-0.491μg（r=0.9990）与峰面积的线性关系良好,回收率分别为102.40%、98.41%,RSD分别为2.25%、1.87%（n=6）。结论所用方法简便、快捷、准确,可用于赤胫散药材的质量控制。OBJECTIVE To establish an HPLC method for the determination of 3,3＇ - di - 0 - methyl ellagic acid and 3,3＇ ,4＇ - trimethyl ellagic acid in Polygonum runcinatum. METHODS Dikma - Cl8 column column and methyl - acetonitrile - 0. 1% phosphoric acid（ 17 ： 33 ： 50） mobile phage were used. The flow rate was 1 mL.min-1 arid the detection wavelength was 248 nra. The column temperature was 30 ℃. RESULTS The linear ranges of 3,3＇ - di - 0 - methyl ellagic acid and 3,3＇ ,4＇ - trimethyl ellagic acid were 0. 198 -0. 990 p,g（ r = 0. 9991 ） and 0. 098 - 0. 491 μg（0. 9990） , respectively. The average recoveries were 102.40% （RSD = 2.25% , n = 6 ） and 98.41% （ RSD = 1.87% , n = 6 ） , respectively. CONCLUSION The method is simple, rapid and accurate, and can be used for the quality control of P. runcinatum.

关 键 词：赤胫散 3 3'-二甲基鞣花酸 3 3' 4'-三甲基鞣花酸 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]