HPLC同时测定半夏中的3种核苷  

Determination of three nucleosides in Pinellia ternata by HPLC

作　　者：孙全[1] 何丹[1] 傅亚[2] 秦少容[3] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆400016 [2]重庆科技学院化学化工学院,重庆401331 [3]太极集团重庆涪陵制药厂有限公司,重庆408000

出　　处：《华西药学杂志》2015年第2期242-244,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家科技支撑计划资助项目(2011BAI13B03)

摘　　要：目的采用HPLC测定半夏中尿苷、鸟苷和腺苷的含量。方法采用Hypersil ODS（5μm,4.6 mm×250 mm）色谱柱,柱温30℃,流动相为甲醇-水,梯度洗脱0-17 min（3.5%-100%甲醇）,检测波长254 nm,流速1 m L·min^-1。结果尿苷、鸟苷、腺苷分别在6.19-43.33、5.09-35.63、1.37-9.60μg·m L-1与峰面积的线性关系良好,线性方程分别为：Y=40.8110X-1.0926（r=0.9999）,Y=49.5173X＋2.1714（r=0.9999）,Y=49.5006X＋0.1857（r=0.9999）,尿苷、鸟苷、腺苷平均加样回收率分别为97.83%、97.15%、98.08%,RSD分别为0.89%、0.69%、1.36%。结论所用方法准确、快速、可靠,能有效地测定半夏中的尿苷、鸟苷、腺苷。OBJECTIVE To establish an HPLC method for the determination of uridine, guanosine, and adenosine in Pinellia ternata. METHODS The Hypersil ODS C18 analytical column（250 mm ×4.6 mm,5 μm） was used and the column temperature was maintained at 30 ℃. The mobile phase was methanol and water with the gradient elution （0 - 17 min, 3.5% - 100% methanol）. The detection wavelength was 254 nm. The flow rate was 1 mL· min^ -1. RESULTS The method was developed with a good linearity with the range of 6, 19 - 43.33 μg· mL^- 1 for uridine ,5.09 - 35.63 μg·mL^-1 for guanosine, and 1.37 - 9.61 μg· mL^ -1 for adenosine. The regression equation of uridine, guanosine and adenosine were as follows ： Y = 40. 8110X- 1. 0926 （r = 0. 9999 ）, Y = 49. 5173X ＋ 2. 1714 （r = 0. 9999 ）, Y = 49. 5006X ＋ 0. 1857 （r = 0. 9999 ） and the average recoveries were 97.83% , 97.15 % , 98.08% and RSD were 0. 89% ,0.69% , 1.36% , respectively. CONCLUSION The method is accurate and rapid. It can be used for the determination of nucleosides in Pinellia ternata.

关 键 词：半夏 尿苷 鸟苷 腺苷 高效液相色谱法 核苷 梯度洗脱 

分 类 号：R917[医药卫生—药物分析学]