流动注射化学发光法测定兰索拉唑的研究  

Determination of lansoprazole by flow injection chemiluminescence system

作　　者：马媛[1] 张琰图[1] 刘宏美[1] 刘欢[1] 双海军[1] 

机构地区：[1]延安大学化学与化工学院,延安716000

出　　处：《分析试验室》2015年第4期442-446,共5页Chinese Journal of Analysis Laboratory

基　　金：陕西省高水平大学建设专项资金(2013SXTS03)资助

摘　　要：兰索拉唑在Ag NPs-Luminol-K3Fe（CN）6化学发光体系中能够产生强烈的化学发光信号,结合流动注射分析技术,提出了检测兰索拉唑的化学发光新方法。在实验条件下,本法测定兰索拉唑的线性范围为3.0×10-3～1.5 mg/L,检出限为2.0×10-4mg/L（3σ）,相对标准偏差为1.5%（n=11,c=3.0×10-2mg/L）。方法用于片剂及人血清中兰索拉唑的含量测定,回收率为98.0%～102.5%。同时,对可能的化学发光反应机理进行了初步探讨。A flow injection chemiluminescence（ CL） method for the determination of lansoprazole was proposed based on the phenomenon that lansoprazole can obviously enhance the CL singal in Ag NPs-Luminol-K3Fe（ CN）6system. Under the experimental conditions,a linear relationship was obtained between the CL intensity and the concentration of lansoprazole over the range from 3. 0 × 10- 3～ 1. 5 mg / L with a detection limit of 2. 0 ×10- 4mg / L（ 3σ）. The relative standard deviation was 1. 5% for 3. 0 × 10- 2mg / L lansoprazole solution in 11 repeated measurements. The proposed method was applied to the analyze lansoprazole in tablets and human serum,respectively with the recovery of between 98. 0% ～ 102. 5%. The possible mechanism of this CL reaction was also proposed.

关 键 词：兰索拉唑 化学发光 纳米银 流动注射 

分 类 号：O657.3[理学—分析化学]