3'-大豆苷元磺酸钠对脑缺血/再灌注损伤大鼠血脑屏障结构及通透性的影响  

Effect of 3'-Daidzein Sulfonate Sodium on Blood-Brain Barrier and Permeability of Cerebral Ischemia/Reperfusion Injured Rats

作　　者：曾靖[1,2] 单热爱[3,2] 余海[3,2] 魏桂林[3,2] 刘宗亮[1] 黎晓[1,2] 李良东[1,2] 

机构地区：[1]赣南医学院 [2]江西省脑血管药理重点实验室,江西赣州341000 [3]赣南医学院第一附属医院

出　　处：《赣南医学院学报》2015年第1期6-8,13,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金资助项目(81160399);江西省高等学校科技落地计划项目(科学前沿;NO:KJLD13085)

摘　　要：目的：观察3＇-大豆苷元磺酸钠对脑缺血／再灌注损伤大鼠血脑屏障结构及通透性的影响。方法：采用MCAO方法制备脑缺血再灌注大鼠模型，并通过尾静脉给予3＇-大豆苷元磺酸钠治疗，采用静脉注射伊文思蓝观察血脑屏障通透性的改变，采用透射电镜观察血脑屏障超微结构的改变。结果：3＇-大豆苷元磺酸钠可显著改善血脑屏障通透性，表现为降低伊文思蓝在脑中的分布，血脑屏障超微结构显示3＇-大豆苷元磺酸钠可显著提高血脑屏障的完整性，能显著减轻血管周围星型胶质细胞和周细胞的肿胀，保持血管的正常结构。结论：3＇-大豆苷元磺酸钠在0．5～2．0 mg·kg^－1范围内可显著保护脑缺血／再灌注损伤大鼠引发的血脑屏障损伤。Objective：To observe how the 3＇-daidzein sulfonate sodium （DSS）affects the blood brain barrier （BBB） structure and permeability in cerebral ischemia/reperfusion injured rats.Methods：The method of Middle cerebral artery occlusion（MACO）was employed to establish the cerebral ischemia/reperfusion injury model of rats.After administrating DSS and Evans blue（EB）by tail vein injection,the ultrastructure changes were observed by transmission electron micro-scope （TEM）.Results：The distribution of Evans blue in brain was decreased by injecting DSS.The blood-brain barrier ultrastructure showed that the integrity of the blood-brain barrier in rat was significantly increased in DSS treating group, concomitantly with release of the swell of astrocyte and pericyte in Virchow-Robin.Conclusion：DSS at dose ranging from 0.5 mg·kg^-1 to 2.0 mg·kg^-1 can significantly protect the BBB injury induced by cerebral ischemia/reperfusion.

关 键 词：3'-大豆苷元磺酸钠 脑缺血/再灌注损伤 血脑屏障 

分 类 号：R285[医药卫生—中药学]