100Hz电针刺激对脑组织和脾脏乙酰胆碱酯酶活性的影响  

Effect of 100 Hz Electroacupuncture on the Activities of Acetycholinesterase in Brain Tissue and Spleen of Mice

作　　者：欧阳娟[1] 林乐迎[2] 邹晓琴[1] 黄诚[1,3] 

机构地区：[1]赣南医学院生理学教研室,江西赣州341000 [2]赣南医学院人体解剖学教研室,江西赣州341000 [3]江西省脑血管药理重点实验室,江西赣州341000

出　　处：《赣南医学院学报》2015年第1期18-20,165,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(31060144);江西省自然科学基金项目(2008GZY0067)资助

摘　　要：目的:观察100 Hz电针刺激对脑组织和脾脏乙酰胆碱酯酶活性影响,初步探讨电针的免疫功能调节作用机制。方法:将昆明小鼠24只随机分成对照组、插针不电组和电针组。给予小鼠电针4天后处死,分别检测小鼠血清、延髓、下丘脑和脾脏中乙酰胆碱酯酶(ACh E)活性。结果:与对照组比较,100 Hz电针刺激可显著降低小鼠血清、延髓、下丘脑和脾脏中乙酰胆碱酯酶(ACh E)活性(P<0.05)。结论:胆碱能抗炎通路可能参与了100 Hz电针调节免疫功能的作用。Objective：To observe the effect of 100 Hz electroacupuncture on the activities of AChE in brain tissue and spleen and to investigate the immunoregulation effect and mechanism of electroacupuncture treatment.Methods：24 male KM mice were randomly divided into normal control group,non-electro acupuncture group,and electro-acupuncture group with 8 in each group.On the fourth day of electroacupuncture,the mice were sacrificed and the specimen of serum,me-dulla,hypothalamus and spleen were harvested to evaluate the activities of AChE.Result：Compared with the normal group,the activity of AChE in serum,medulla,hypothalamus and spleen in electroacupuncture group decreased obvious-ly （P〈0.05）.Conclusion：100 Hz electroacupuncture may regulate immune function via cholinergic anti-inflammatory pathway.

关 键 词：电针 免疫调节 乙酰胆碱酯酶 胆碱系统 

分 类 号：R245[医药卫生—针灸推拿学]