100Hz电针刺激对脑组织和脾脏乙酰胆碱酯酶mRNA表达的影响  

Effect of 100 Hz Electroacupuncture on the mRNA Expressions of ACh E of the Brain Tissue and Spleen

作　　者：欧阳娟[1] 邹晓琴[1] 黄诚[1,2] 

机构地区：[1]赣南医学院生理学教研室 [2]江西省脑血管药理重点实验室,江西赣州341000

出　　处：《赣南医学院学报》2015年第1期21-23,165,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家自然科学基金项目(31060144);江西省自然科学基金项目(2008GZY0067)资助

摘　　要：目的:观察100 Hz电针刺激对脑组织和脾脏乙酰胆碱酯酶mRNA表达的影响,初步探讨电针的免疫功能调节作用机制。方法:将昆明小鼠24只随机分成对照组、插针不电组和电针组。给予小鼠电针4天后处死,用RT-PCR方法分别检测小鼠血清、延髓、下丘脑和脾脏中乙酰胆碱酯酶(ACh E)mRNA表达情况。结果:RT-PCR显示,小鼠血清、延髓、下丘脑和脾脏中均有乙酰胆碱酯酶mRNA的表达。结论:胆碱能抗炎通路可能参与了100 Hz电针调节免疫功能的作用。Objective：To observe the effect of 100 Hz electro-acupuncture on the mRNA expressions of AChE and to in-vestigate the immunoregulation effect and mechanism of electro-acupuncture treatment.Methods：24 male KM rats were randomly divided into normal control group,non-electro acupuncture group,and electro-acupuncture group with 8 in each group.On the fourth day of electro-acupuncture,the rats were sacrificed and RNA was extracted by kit from the serum, medulla,hypothalamus,and spleen to detect the expressions of AChE by RT-PCR.Result：RT-PCR showed that AChE was expressed in serum,medulla,hypothalamus,and spleen.Conclusion：100 Hz electroacupuncture may regulate im-mune function via cholinergic anti-inflammatory pathway.

关 键 词：电针 免疫调节 胆碱能系统 乙酰胆碱酯酶 

分 类 号：R245[医药卫生—针灸推拿学]