亚甲基四氢叶酸还原酶基因多态性与先兆流产相关性研究  

Study on the Correlation between Genetic Polymorphism of Methylenetetrahydrofolate Reductase and Threatened Abortion

作　　者：黄晓玲[1] 何艳君[1] 鲁衍强 李瑛 林云霞[1] 

机构地区：[1]中山市博爱医院,广东中山528403 [2]上海靶向分子医学研究所,上海200433

出　　处：《赣南医学院学报》2015年第1期41-43,47,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：中国疾病预防控制中心妇幼保健中心妇幼保健分子遗传医学研究专项计划(No:FY-ZX-ZD-0066);中山市科技局科研立项课题(编号:20132A009)

摘　　要：目的:探讨先兆流产与亚甲基四氢叶酸还原酶(MTHFR)基因多态性的关联性。方法:采用病例对照研究的方法,以91例先兆流产者和116例正常妇女为研究对象,利用荧光定量PCR技术,检测受检者MTHFR基因C677T、A1298C位点的单核苷酸多态性(SNP)。结果:病例组MTHFR 677TT基因型的频率为15.4%,显著高于对照组(P<0.05);病例组MTHFR 1298CC基因型的频率为5.5%,显著高于对照组(P<0.05),且MTHFR A1298C位点的分布情况在两组间差异有统计学意义(P<0.05)。结论:MTHFR C677T和A1298C基因多态性变化与先兆流产的发生具有一定的相关性。Objective：To explore the correlation between gene polymorphisms of methylenetetrahydrofolate reductase （MTHFR）and threatened abortion.Methods：Case control study was used.91 cases of threatened abortion and 116 normal women were taken as research objects.The single nucleotide polymorphism （SNP）of their MTHFR gene polymor-phisms were detected by using fluorescence quantitative PCR.Results：The frequency of MTHFR 677TT in patients （15.4%）was obviously higher than those in control group （P〈0.05）;the frequency of MTHFR 1298CC in patients （5.5%）was obviously higher than those in control group （P〈0.05）;MTHFR A1298C genotype frequency distribution in patients was different from those in controls （P〈0.05）.There had statistically significant differences.Conclusion：Genetic polymorphism of MTHFR C677T and A1298C changes in correlation with the occurrence of threatened abortion.

关 键 词：先兆流产 亚甲基四氢叶酸还原酶 单核苷酸多态性 

分 类 号：R714.21[医药卫生—妇产科学]