玫瑰黄链霉菌片剂对根结线虫病的防治作用及对土壤微生物区系的影响  

Control efficacy of Streptomyces roseoflavus tablets on cucumber root-knot nematode and its effect on soil microorganisms

作　　者：乔丹娜[1] 李华义[1] 韩兴[1] 张艳杰[1] 沈凤英[1] 刘大群[1] 李亚宁[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《农药学学报》2015年第2期163-171,共9页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(31171894);国家高技术研究发展计划("863"计划)项目(2011AA10A205);河北省自然科学基金(C2011204114)

摘　　要：玫瑰黄链霉菌Men-myco-93-63是从马铃薯疮痂病自然衰退土壤中筛选到的对多种重要植物病原菌具有较强抑制作用的拮抗链霉菌,其发酵液对根结线虫具有较好的防治作用。为明确Men-myco-93-63活菌片剂在蔬菜根结线虫防治中的应用前景,采用温室盆栽法和稀释平板法研究了Men-myco-93-63活菌片剂对黄瓜根结线虫病的防治效果及对土壤微生物区系的影响。结果表明:Men-myco-93-63片剂对黄瓜根结线虫病具有较好的防效,1.0和1.5 g/株用量下防效分别为51.85%和55.56%,而对照药剂淡紫拟青霉颗粒剂1.5 g/株及M en-myco-93-63固体发酵物(发酵物与病土体积比为1∶200)的防效分别为54.17%和50.00%;施药60d后,该片剂对植株根际土壤中微生物区系影响较大,1.0 g/株用量下,细菌和放线菌数量分别比空白对照增加了225.45%和75.45%,真菌数量降低了66.43%,3种可培养微生物的总量比空白对照增加了195.35%,表明M en-myco-93-63片剂的施用可明显改善根际土壤中的微生物结构。Streptomyces roseoflavus Men-myco-93-63 is a biological agent isolated from potato scab disease declined soil and has evident antagonistic action against many plant pathogens, including root-knot nematode. In order to evaluate the application prospects of Men-myco-93-63 tablets in controlling root-knot nematode, greenhouse pot experiment and dilution-plate method were conducted to determine the control efficacy of the tablets on cucumber root-knot nematode and its effect on soil microorganisms. The results showed that the control efficacy were 51. 85% and 55. 56% after treatment on cucumber by 1. 0 g/plant and 1. 5 g/plant, respectively, while that of the control agent-Paecilomyces lilacinus and solid fermentation of Men-myco-93-63 were 54. 17% and 50. 00%, respectively. Men-myco-93-63 tablets had marked effect on soil microorganisms after 60 days, the number of bacteria and actinomyces increased by 225. 45% and 75. 45% respectively, and the number of fungi reduced by 66. 43% after treatment on cucumber by 1. 0 g/plant. The total number of the 3 kinds of soil microorganisms increased by 195. 35%, suggesting the tablets could significantly improve the structure of soil microorganisms.

关 键 词：玫瑰黄链霉菌 MEN-MYCO-93-63 根结线虫 片剂 防治效果 土壤微生物 

分 类 号：TQ453.5[化学工程—农药化工] S432.45[农业科学—植物病理学]