多胎和单胎山羊品种促性腺素基因及其受体基因的克隆、序列分析及组织表达研究  

Cloning and expression levels of gonadotropin genes and receptor genes in polytocous and monotocous goat breeds

作　　者：胡亮[1] 字向东[1] 卢建远[1] 马力[1] 熊显荣[1] 王永[1] 任称罗尔日 任陶 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省理县畜牧局,四川理县623100

出　　处：《西南民族大学学报（自然科学版）》2015年第2期138-143,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省应用基础项目(2013JY0043);中央高校基本科研业务费专项资金项目(2014ZYXS55)

摘　　要：以多胎的金堂黑山羊和单胎的藏山羊为研究对象,通过RT-PCR技术,对金堂黑山羊和藏山羊FSHβ、LHβ、FSHR和LHR基因进行克隆,采用Real-time PCR技术测定FSHR和LHR基因在发情前期母羊卵巢中的表达量,采用酶联免疫法测定血浆中FSH和LH的浓度.结果表明:金堂黑山羊和藏山羊FSHβ、LHβ、FSHR和LHR基因的CDS区长度分别为390bp、426bp、2088bp、2103bp,同源性分别为99.74%、100%、99.95%、99.57%;发情前期两山羊品种之间卵巢FSHR和LHR基因的mRNA相对表达量差异不显著(P>0.05),血浆中FSH浓度无显著差异(P>0.05),LH的浓度差异显著(P<0.05).提示FSHβ和LHR基因序列及血浆中LH浓度在两个品种间的差异可能是品种间产羔数性状差异的重要原因.This study was carried out to clone the sequences of FSHβ, LHβ, FSHR and LHR cDNAs of prolific Jintang black gnat and nonprnlific Tibetan goat by RT-PCR. FSHR and LHR mRNA expressions during proestrus in ovary were determined by real-time PCR, and plasma concentrations of FSH and LH were determined by ELISA. The result showed that the coding region of goat FSHβ, LHβ ,FSHR and LHR genes were 390bp ,426bp ,2088bp and 2103bp long, respectively. The homologies of coding region of FSHβ, LHβ, FSHR and LHR genes between Jintang black goat and Tibetan goat were 99. 74%, 100% ,99. 95%, 99. 57%. The FSHR and LHR mRNA were expressed in ovary of the two goat breeds during proestrus, but there was no significant difference between the two breeds （P 〉0. 05 ）. There was no significant difference between the two breeds in plasma concentrations of FSH （ P 〉 0.05 ）, but the concentrations of LH had significant difference （ P 〈 0. 05 ）. The results suggest that differences in gene sequences of FSHβ and LHR, and plasma concentration of LH between the two breeds may be associated with the differences in their kidding rates.

关 键 词：金堂黑山羊 藏山羊 克隆 促性腺素 实时PCR 

分 类 号：S814[农业科学—畜牧学] S827[农业科学—畜牧兽医]