青春期雄性小鼠双酚A暴露对生殖功能及子代性别比的影响  

Adolescent exposure to bisphenol A affects the reproduction of male mice and sex ratio of offsprings

作　　者：李玉华[1,2] 段斐[2] 杨芬[2] 周小羽[2] 潘鸿捷[2] 李扬[1] 李润生[2] 

机构地区：[1]吉林大学白求恩医学院病理生理学教研室,长春130021 [2]国家卫生与计划生育药具重点实验室,上海市计划生育科学研究所,上海200032

出　　处：《生殖与避孕》2015年第3期141-145,165,共6页Reproduction and Contraception

基　　金：国家自然科学基金资助项目;基金编号:81270760

摘　　要：目的:研究双酚A(BPA)暴露对青春期雄性小鼠成年后生殖功能及对子代的影响。方法:21日龄C57BL/6J雄性小鼠每日腹腔注射BPA 50 mg/kg连续7 d,35 d后检测成年后雄鼠附睾尾精子数量、精子畸形率、睾丸组织学变化;与正常雌性小鼠配种,观测生育力指标以及仔鼠的出生情况。结果:BPA暴露能引起小鼠附睾尾精子数量下降20.6%(P<0.01);精子畸形率增加9.65%(P<0.05);睾丸组织结构异常;BPA暴露对雄性小鼠成年后的生育力没有明显影响;但能引起子代雄∶雌出生性别比升高。结论:青春期雄鼠BPA暴露能引起成年雄鼠生精功能下降,仔小鼠雄性比例增加。Objective： To study the effects of adolescent exposure to bisphenol A （BPA） on the reproduction of male mice in adulthood and subsequent generation mice. Methods： Male mice aged 21 d were exposed to BPA at a dose of 50 mg/kg per day for 7 d by intraperitoneal injection. Sperm count, sperm deformity rate and testis histology were evaluated 35 d after exposure. Male fertility index and newborns were further observed by mating with the normal female mice. Results： The epididymal sperm number was decreased by 20.6% in BPA exposure group compared with the control （P〈0.0 1）. Sperm deformity rate in BPA group was increased by 9.65% compared with the control （P〈0.05）. Testis seminiferous tubules were abnormal with sloughing of germ cells; BPA exposure had no significant effects on the fertility of male mice in adulthood; sex ratio of male to female offspring was increased. Conclusion： Adolescent exposure to BPA can disrupt spermatogenesis in adulthood, and the proportion of male offspring was increased compared with the control.

关 键 词：双酚A(BPA) 生殖功能 性别比 

分 类 号：R114[医药卫生—卫生毒理学]