二甲基亚砜通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎  

Dimethyl sulfoxide alleviates protamine sulfate-induced rat cystitis by reducing inflammation and oxidative stress

作　　者：林斌[1] 董兴有 赵江[1] 王亮[1] 龙州 朱景振 丁国林[1] 李龙坤[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科,重庆400037

出　　处：《第三军医大学学报》2015年第6期505-509,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81170704)~~

摘　　要：目的探讨二甲基亚砜(dimethyl sulfoxide,DMSO)对鱼精蛋白(protamine sulfate,PS)诱导的大鼠间质性膀胱炎(interstitial cystitis,IC)治疗作用。方法 SD雌性大鼠48只,按随机数字表法分为正常组、PS灌注组、PS+DMSO组、盐水灌注组(NS组),每组12只大鼠。正常组大鼠未给予任何处理;PS组用0.5 m L,30 mg/m L的PS膀胱内灌注并在膀胱内保留30 min;PS+DMSO组使用50%DMSO溶解PS,按照0.5 m L,30 mg/m L的PS膀胱内灌注并在膀胱内保留30 min;NS组用0.5 m L生理盐水膀胱灌注并保留30 min,各组大鼠每周处理1次,连续处理4周。4周后,HE和甲苯胺蓝染色用于病理学评分和肥大细胞计数;尿动力学和肌条实验被用于评价大鼠膀胱功能和肌肉收缩性改变;Western blot检测SOD2、GSH-Px、IL-6、IL-1B、TNF-α的表达量。结果与PS组相比,PS+DMSO组大鼠膀胱病理学评分[(3.00±0.00)vs(0.83±0.40),P<0.05]、肥大细胞计数[(19.66±2.16)vs(6.16±1.16),P<0.05]以及炎症因子IL-6、IL-1B和TNF-α表达(P<0.05)显著降低,而抗氧化应激酶(SOD2和GSH)显著恢复(P<0.05);在膀胱功能学上,与PS组相比,PS+DMSO组大鼠膀胱排尿频率[(24.83±3.06)vs(9.83±1.47),P<0.05]显著降低,排尿间隔[(2.42±0.37)vs(6.40±0.57),P<0.05]显著延长,肌肉收缩频率[(6.93±0.97)vs(4.94±0.73),P<0.05]显著降低。结论 DMSO可能通过降低炎症和氧化应激改善鱼精蛋白诱导的大鼠膀胱炎。Objective To investigate the effect of dimethyl sulfoxide （DMSO） on the treatment of protamine sulfate （PS）-induced interstitial cystitis in rats. Methods Totally, 48 SD rats were randomized into a normal group, a PS perfusion group, a PS ＋ DMSO group, and a saline perfusion group （ NS group）, 12 rats in each group. The normal group was not given any treatment. The PS group was treated with 0.5 mL, 30 mg/mL PS by bladder perfusion for 30 rain. The PS ＋ DMSO group was treated with 0.5 mL, 30 mg/mL PS ＋ DMSO （PS dissolved in 50% DMSO） by bladder perfusion for 30 min. The NS group was treated with 0.5 mL normal saline by bladder perfusion for 30 min. The rats were treated once in a week and the treatment lasted for 4 weeks. After 4 weeks, HE staining and toluidine blue staining were used for pathological scoring and mast cell counting. Urodynamics and muscle strip test were used for the evaluation of bladder function and muscle contraction. The changes of superoxide peroxidase 2 （SOD2）, glutathione peroxidase （GSH-Px） , interleukin 6 （ IL-6 ） , interleukin-1β （ IL-1β ） , and tumor necrosis factor-or （TNF-a） were measured by Western blotting. Results Compared with the PS group, the rat bladder pathological scores （3.00 ±0.00 vs 0.83 ±0.40, P 〈 0. 05）, mast cell count （ 19.66 ± 2.16 vs 6.16± 1.16, P 〈 0. 05 ）, and expression of inflammatory cytokines （IL-6, IL-1β and TNF-a, P 〈 0. 05 ） were significantly decreased in the PS ＋ DMSO group, and the antioxidant enzymes （SOD2 and GSH-Px ） were significantly increased （P 〈 0. 05 ）. For bladder function, compared with the PS group, the PS ＋ DMSO group showed lower micturition frequency （24. 83±.06 vs 9.83±1.47, P 〈0. 05）, shorter intereontraction interval （2.42±0. 37 vs 6.40 ± 0.57, P 〈 0. 05 ）, and lower muscle contraction frequency （6.93 ＋ 0.97 vs 4.94 s- 0.73, P 〈 0. 05 ）. Gonelusion DMSO alleviates the PS-induced rat cystitis by reducing inflammation and oxidative str

关 键 词：二甲基亚砜 间质性膀胱炎 鱼精蛋白 膀胱炎 

分 类 号：R694.305[医药卫生—泌尿科学] R965[医药卫生—外科学]