银川地区某奶牛场牛支原体病的诊断  

Diagnosis of Mycoplasma bovis in a cattle farm of Yinchuan

作　　者：郭澍强 蒋万[2] 罗海峰[1] 葛松[1] 王静[1] 杨萌萌[1] 何生虎[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏大学实验农场,宁夏银川750021

出　　处：《中国预防兽医学报》2015年第3期203-206,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：宁夏科技攻关重点专项(412-0164)"肉牛呼吸疾病综合症及皮肤病防控技术研究与示范"

摘　　要：为确诊银川市某奶牛场成年奶牛病因,本研究对两头表现明显临床症状的犊牛进行解剖,无菌采集肺脏、肺积液、关节液及患临床型乳房炎的奶牛乳样1份进行病原分离,分离到2株巴士杆菌、2株链球菌、1株大肠杆菌和3株支原体,通过革兰氏染色、菌落形态观察、特异性PCR鉴定和16S r RNA序列比对,结果显示引起此次流行的主要病原为牛支原体。采用比利时进口牛支原体ELISA诊断试剂盒对银川地区12个规模化奶牛场的230份奶牛血清进行牛支原体血清学调查,结果显示牛群平均阳性率26.52%,牛场阳性率高达100%,表明牛支原体病在银川地区呈高感染率。A case emerged in a cattle farm of Yinchuan in November 2013, which caused pneumonia and mastitis in cows and breathing difficult and arthritis in calf. By Gram stain, colony morphology, specific PCR identification and 16S rRNA sequence alignments proved the cows mainly caused by the pathogen Mycoplasma bovis. Moreover, a total of 230 serum samples from 12 large-scale dairy farm were examined using commercial ELISA, the result showed that the positive rate was 26.52% for M.bovis and all the farm had the M. bovis infection, which indicated that the M. boris is commonly prevalence in Yinchuan.

关 键 词：牛支原体 分离与鉴定 血清学调查 

分 类 号：S852.62[农业科学—基础兽医学]