恶性疟原虫裂殖子与人血型糖蛋白A之间分子识别的研究  

MOLECULAR RECOGNITION BETWEEN GLYCOPHORIN A AND PLASMODIVM FALCIPARUM MEROZOITES

作　　者：骆训懿[1] 王向东[1] 李建新[1] 魏尧梅[1] 郑尊[2] 严永碧 

机构地区：[1]第二军医大学生化教研室 [2]第二军医大学电镜室

出　　处：《中国寄生虫学与寄生虫病杂志》1991年第1期1-3,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：用纯化的人血型糖蛋白A(Glycophorin A,简称GPA)和GPA糖肽分别对恶性疟原虫FCC-1/HN株的裂殖子进行免疫胶体金标记。标记样品按常规电镜要求处理,透射电镜观察。结果显示,胶金颗粒呈弥漫状分布于整个裂殖子虫体表面,获得恶性疟原虫裂殖子与GPA及GPA糖肽特异识别结合的直观实验证据,属国内外首次报道。本结果进一步证实GPA是恶性疟原虫裂殖子的受体,及GPA糖肽区域是膜受体的活性部位。By using purified human erythrocyte membrane glycophorin A (GPA) and glycopeptide of GPA, antibodies against GPA and against GPA- glycopeptide, and SPA-colloidal gold, Plasmodium falciparum FCC-l/HN merozoites were immunolabeled. The labeled samples were observed under transmission electron microscope (TEM). The TEM pictures showed that colloidal gold pellets were distributed over all of the merozoite surface. This is the first report on the direct experimental evidence of molecular recognition and combination between GPA (or glycopeptide of GPA) and Plasmodium falciparum merozoites. The results strongly support the hypothesis that GPA is involved as a recognized receptor on erythrocy-tes for Plasmodium falciparum and the glycopeptide domain of GPA is the receptor site for Plasmodium falciparum.

关 键 词：恶性疟原虫 血型糖蛋白A 电镜 

分 类 号：R382.31[医药卫生—医学寄生虫学]