本经取穴针刺法对肝阳上亢型无先兆偏头痛患者MME基因表达的影响  

Effect of Acupuncture along Affected Meridian on the MME Gene Expression of Migraine Patients without Aura of Gan-yang Hyperactivity Syndrome

作　　者：黄银兰[1] 万明雨[2] 梁希森[1] 梁繁荣[3] 

机构地区：[1]宁夏医科大学中医学院,银川750003 [2]北京中医药大学东方学院,河北065001 [3]成都中医药大学针推学院,成都610075

出　　处：《中国中西医结合杂志》2015年第3期294-298,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30960485);国家重点基础研究发展计划(973计划)资助项目(No.2012CB518500)

摘　　要：目的观察本经取穴针刺法调控肝阳上亢型无先兆偏头痛患者膜金属肽链内切酶(membrane metallo-endopeptidase,MME)基因的差异表达。方法将20例肝阳上亢型无先兆偏头痛患者随机分为经穴组(针刺患侧经穴)和非经非穴组(针刺患侧非经非穴),每组各10例,每天针刺1次,连续10天。利用基因芯片技术,获得两组的基因表达谱,并对其进行Gene Ontology(GO)分析。结果与本组治疗前比较,经穴组治疗后MME基因表达量下降(P=0.0023),该基因富集于β-淀粉样蛋白代谢过程(beta-amyloid metabolic process,P=3.16E-05)以及肽代谢过程(peptide metabolic process,P=0.009612),该基因在非经非穴组治疗后各样本中未见表达。结论本经取穴对肝阳上亢型无先兆偏头痛的效应可能通过对MME基因的调控实现。Objective To observe the effect of acupuncture along affected meridian on the mem- brane metallo-endopeptidase （MME） gene expression of migraine patients without aura （MO） of Gan- yang hyperactivity syndrome （GYHS）. Methods Totally 20 MO patients of GYHS were randomly as- signed to the acupoint group （acupuncture along affected meridian） and the non-acupoint group, 10 ca- ses in each group. Needling was performed once per day for 10 consecutive days. Gene chip technology was used to obtain two sets of gene expression profiles and analyzed using Gene Ontology （GO）. Results In the acupoint group, MME gene expression decreased after needling （P =0.0023）. That gene was rich in the beta-amyloid metabolic process （P =3.16E -05） and the peptide metabolic process （P = 0.009612）. Its expression was not seen in the non-acupoint group. Conclusion The effect of point selec- tion along affected meridian could be achieved possibly by regulating the MME gene expression.

关 键 词：本经取穴 膜金属肽链内切酶基因 脑啡肽酶 无先兆偏头痛 

分 类 号：R246.6[医药卫生—针灸推拿学]