电针内关穴及列缺穴对心肌缺血大鼠心肌细胞蛋白激酶表达的影响  

Effect of Electro-acupuncture at Neiguan( PC6) and Lieque( LU7) on the Expression of Protein Kinases in Cardiomyocytes of Myocardial Ischemia Rats

作　　者：王颖[1] 李迪[1] 戴俭宇[1] 刘昱甫 荆秦[1] 王熙[1] 王列[1] 

机构地区：[1]辽宁中医药大学针灸推拿学院针灸临床教研室,沈阳110032

出　　处：《中国中西医结合杂志》2015年第3期338-342,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家973计划资助项目(No.2012CB518500)

摘　　要：目的研究电针内关穴及列缺穴对心肌缺血大鼠心肌细胞蛋白激酶表达的影响。方法健康雄性SD大鼠按照随机数字表随机分为对照组、模型组、内关组、列缺组、非经非穴组,每组10只。采用注射盐酸异丙肾上腺素(85 mg/kg)制作大鼠心肌缺血模型,针刺内关、列缺、非经非穴进行干预,通过Western blot检测技术,观察电针前后各组大鼠心肌细胞蛋白激酶A(protein kinase A,PKA)、蛋白激酶C(protein kinase C,PKC)及蛋白激酶G(protein kinase G,PKG)表达量的变化情况。结果与对照组比较,模型组PKA、PKC、PKG的表达明显升高(P<0.01)。与模型组比较,内关组和列缺组PKA、PKC、PKG蛋白表达量均降低(P<0.01,P<0.05),非经非穴组PKA蛋白表达量降低(P<0.01);与内关组比较,列缺组、非经非穴组PKA、PKC、PKG的表达量明显升高(P<0.01,P<0.05);与列缺组比较,非经非穴组PKA、PKC、PKG表达量升高(P<0.01,P<0.05)。结论电针内关穴和列缺穴均可以降低心肌缺血大鼠心肌细胞蛋白激酶的表达量,且内关穴的针刺效应优于列缺穴。Objective To study the effect of electro-acupuncture （EA） at Neiguan （PC6） and Lieque （LU7） on the expression of protein kinases in cardiomyocytes of myocardial ischemia （MI） rats. Methods Healthy male SD rats were randomly divided into the control group, the model group, the Neiguan point group, the Lieque point group, and the non-meridian non-acupoint group, 10 in each group by random digit table. The MI rat model was established by injecting isoprenaline hydrochloride （85 mg/kg）. EA at Neiguan （PC6）, Lieque （LU7）, and non-meridian non-acupoint were respectively per- formed. Changes of the expression of protein kinases [such as protein kinase A （PKA）, protein kinase C （PKC）, protein kinase G （PKG）] in rat cardiomyocytes were observed using Western blot. Results Compared with the control group, expression levels of PKA, PKC, and PKG increased obviously in the model group （P 〈0.01 ）. Compared with the model group, expression levels of PKA, PKC, and PKG de- creased in the Neiguan point group and the Lieque point group （P 〈0.01, P 〈0.05）. Expression levels of PKA decreased in the non-meridian non-acupoint group （P 〈 0.01 ）. Compared with the Neiguan point group, expression levels of PKA, PKC, and PKG increased in the non-meridian non-acupoint group and the Lieque point group （P 〈0.01, P 〈0.05）. Compared with the Lieque point group, expression levels of PKA, PKC, and PKG increased in the non-meridian non-acupoint group （P 〈0.01, P 〈0.05）.. Conclusion EA at Neiguan （PC6） and Lieque （LU7） could decrease protein expression levels of PKA, PKC and PKG in rat myocardial cells, and the effect of acupuncture at Neiguan （PC6） was better than that ob- tained by EA at Lieque （LU7）.

关 键 词：心肌缺血 蛋白激酶A 蛋白激酶C 蛋白激酶G 内关穴 列缺穴 

分 类 号：R245[医药卫生—针灸推拿学]