肝素结合性表皮生长因子在翼状胬肉中的表达及意义  

Expression of HB-EGF in human pterygium and its significance

作　　者：邹晶[1] 喻理[1] 许博涵[1] 邵毅[2] 易敬林[1] 

机构地区：[1]南昌大学附属眼科医院,江西省南昌市330046 [2]南昌大学第一附属医院眼科,江西省南昌市330006

出　　处：《眼科新进展》2015年第3期214-217,222,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号81160118;81400372);全国临床医药研究专项基金(编号:L2012052);江西省远航工程项目(编号:2014092);江西省科技平台建设项目(编号:2013116);江西省青年科学基金(编号:20114BAB215036);江西省科技支撑计划项目(编号:20111BBG70026-2);江西省卫生厅中医药科研项目(编号:2012A087);江西省教育厅科技计划项目(编号:GJJ14170);江西省卫计委科技计划项目(编号:20155154)~~

摘　　要：目的研究肝素结合性表皮生长因子(heparin-binding epidermal growth factor,HB-EGF)在翼状胬肉组织中的表达与分布,探讨其在翼状胬肉发生过程中的作用。方法收集2013年8月至2014年8月在南昌大学眼科医院行翼状胬肉切除的40例标本,采用免疫组织化学染色和Western blot分别检测22例初发与18例复发翼状胬肉标本中HB-EGF的表达情况,并与正常对照组结膜标本进行比较。结果HB-EGF在初发型翼状胬肉组织的头部相对表达值为2.77、体部相对表达值为1.76,正常结膜相对表达值为1.37。复发型胬肉组织中HB-EGF在其头部相对表达值为1.95、体部相对表达值为1.57,正常结膜相对表达值为0.96。HB-EGF在初发型和复发型翼状胬肉组织中均主要定位于上皮细胞和血管内皮细胞的胞浆中,以上皮细胞表达最为显著。相同标本数复发型翼状胬肉头部组织中HB-EGF的相对表达值为4.30,为正常对照结膜(相对表达值为1.09)的3.9倍、为初发型(相对表达值为1.32)翼状胬肉头部组织3.1倍,且初发型翼状胬肉头部组织表达量为正常对照组的1.3倍。结论高水平的HB-EGF可能是翼状胬肉发生与复发的重要因素,提示可以把HB-EGF作为干预靶点来控制翼状胬肉的发生与复发。Objective To investigate the expression and distribution of heparinbinding epidermal growth factor（ HB-EGF） in pathogenesis of human pterygium,and study the role of its played. Methods Between August 2013 and August2014, 40 cases of pterygium excision specimens were collected and produced in Affiliated Eye Hospital of Nanchang University. The expression differences of HB-EGF between pterygium and normal conjunctiva tissue from the same pterygium patient were compared with immunohistochemistry and Western blot. Also we used the same technique to examine the expressions and distribution of HG-EGF in pterygium tissue of 22 cases with first operation and 18 recurrent cases as well as the expression in 15 specimens of normal conjunctiva from patients operated for other disorders. Results In primary pterygium group, the relative intensities of HB-EGF in pterygium-head was 2. 77,pterygium-body was 1. 76 and normal conjunctiva tissue was 1. 37. In recurrent pterygium group, the relative intensities of HB-EGF in pterygium-head was 1. 95,pterygium-body was 1. 57 and normal conjunctiva tissue was 0. 96. And HB-EGF largely distributed in the endochylema of epithelial cells and vascular endothelial cells of pterygium, especially in epithelial cells. The relative intensities of total HB-EGF in recurrent group was 4. 30 which was 3. 9 times over the normal conjunctive group（ the relative intensities was 1. 09）,3. 1 times as strong as the primary group（ the relative intensities was1. 32）, and the primary group was 1. 3 times over the normal conjunctive group.Conclusion Over-expression of HB-EGF is an important cause of development and recurrence of pterygium,which indicate that HB-EGF can be set as the therapeutic target for controlling the development and recurrence of pterygium.

关 键 词：翼状胬肉 新生血管 肝素结合性表皮生长因子 

分 类 号：R587.2[医药卫生—内分泌]