正交试验法优选金茵利胆口服液水提工艺  

Optimization of Water Extraction Process for Jinyin Lidan Oral Liquids by Orthogonal Experiment

作　　者：潘新波[1] 李彩东[1] 王信[1] 张伟[1] 

机构地区：[1]兰州市第二人民医院,兰州730046

出　　处：《中国药师》2015年第4期585-588,共4页China Pharmacist

基　　金：甘肃省卫生厅中医药科研项目(编号:GZK-2012-49)

摘　　要：目的:优选金茵利胆口服液的水提工艺。方法:采用正交试验法,以加水量、提取时间、提取次数、浸泡时间为考察因素,出膏率、绿原酸、柚皮苷和新橙皮苷为考察指标,采用高效液相色谱法对绿原酸、柚皮苷和新橙皮苷进行含量测定,以筛选出最佳水提工艺。结果:金茵利胆口服液的最佳水提工艺为:处方量药材加8倍量水,浸泡1 h,煎煮2次,每次1.5 h。结论:该水提工艺稳定、合理,为该制剂的生产工艺和质量控制研究提供研究基础。Objective： To investigate the optimal water extraction process of Jinyin Lidan oral liquids. Methods： An orthogonal design was used in the extraction optimization using the yield and content of chlorogenic acid,narigin and neohesperidin as the evaluation indices and the volume of water,extraction times,extraction time and soaking time as the influencing factors. The contents of chlorogenic acid,narigin and neohesperidin were detected by HPLC to screen the best extraction technology. Results： The best water extraction conditions were as follows： the amount of the added water was 8 times of the medicinal materials,the soaking time was 1 h,and the decoction was carried out twice with the duration of 1. 5 h for each. Conclusion： The process is stable and reasonable,which provides research foundation for the production and quality control.

关 键 词：金茵利胆口服液 正交试验 水提工艺 绿原酸 柚皮苷 新橙皮苷 

分 类 号：R284.2[医药卫生—中药学]