瑞舒伐他汀对脑缺血再灌注损伤大鼠神经细胞凋亡及Caspase-3表达的影响  

Effects of Rosuvastatin on neural cell apoptosis and Caspase- 3 expression of rats with cerebral ischemia reperfusion injury

作　　者：张俊华[1] 苏建华[1] 陈玉芳[2] 薛建琴[1] 耿瑜睿[1] 

机构地区：[1]江苏大学附属金坛医院(金坛市人民医院),江苏金坛213200 [2]江苏省常州市第四人民医院,江苏常州213000

出　　处：《现代中西医结合杂志》2015年第12期1257-1260,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：金坛市科学技术局计划项目(JT2013064)

摘　　要：目的观察瑞舒伐他汀对大鼠脑缺血再灌注损伤神经细胞凋亡及Caspase-3表达的影响,探讨其神经保护作用机制。方法将健康雄性Wistar大鼠随机分为假手术组、模型组、瑞舒伐他汀组,余假手术组外,其余2组制作缺血再灌注损伤大鼠模型。分别在脑缺血再灌注后12 h、24 h、72 h取各组大鼠脑组织,检测神经细胞凋亡数及Caspase-3阳性表达细胞数。结果与假手术组比较,模型组和瑞舒伐他汀组神经细胞凋亡数和Caspase-3阳性表达细胞数均明显增加(P均<0.01),瑞舒伐他汀组神经细胞凋亡数和Caspase-3阳性表达细胞数均明显低于模型组(P均<0.01);随缺血再灌注时间延长,各组神经细胞凋亡数和Caspase-3阳性表达细胞数均逐渐减少,组内再灌注各时间点比较差异均有统计学意义(P均<0.01)。结论瑞舒伐他汀对脑缺血再灌注损伤有神经保护作用,其作用机制可能与下调Caspase-3的表达、对抗细胞凋亡有关。Objective It is to observe the influence of rosuvastatin on nerve cell apoptosis and Caspase- 3 expression of rats with cerebral ischemia reperfusion injury,and investigate the mechanism of neuroprotection. Methods The healthy male Wistar rats were randomly divided into sham operation group,model group and rosuvastatin group,the ischemia reperfusion injury rat model were made in model group and rosuvastatin group respectively. The brain tissue of rats were obtain after cerebral ischemia-reperfusion in 12 h,24 h,and 72 h,and then the number of the apoptosis of neural cells and Caspase- 3 positive cells were measured. Results Compared with the sham operation group,the number of nerve cell apoptosis and Caspase- 3positive cells of the model group and rosuvastatin group were increased significantly（ P 〈0. 01）. Compared with the model group,rosuvastatin significantly reduced the number of Caspase- 3 positive cells,there is significant statistically significant difference（ P 〈0. 01）. Conclusion Rosuvastatin on cerebral ischemia reperfusion injury has neuroprotective effect,and the mechanism may be associated with the down- regulation of Caspase- 3 expression,against cell apoptosis.

关 键 词：瑞舒伐他汀 脑缺血再灌注损伤 神经细胞凋亡 CASPASE-3 

分 类 号：R332[医药卫生—人体生理学]